Document Detail

Different modes of sensory neuropeptides and nitric oxide involvement in relaxation of guinea-pig vessels.
MedLine Citation:
PMID:  8690844     Owner:  NLM     Status:  MEDLINE    
This study investigated involvement of calcitonin-gene-related peptide (CGRP), substance P (SP) and nitric oxide (NO) in transmural nerve stimulation (TNS)-induced non-adrenergic, non-cholinergic (NANC) relaxation in isolated guinea pig anterior mesenteric artery (AMA) and posterior caval vein (PCV). Effects of cyclo-oxygenase-generated eicosanoids were blocked with indomethacin (10(-5) M) and so were adrenergic and cholinergic responses with phentolamine (3 x 10(-6) M), propranolol (10(-6) M) and atropine (10(-6) M). In both vessels precontracted by U-46619, TNS induced relaxation, which was almost completely abolished by capsaicin pretreatment (10(-6) M, 15 minutes). In AMA, a CGRP1 receptor antagonist (human CGRP8-37, 10(-5) M) significantly attenuated the relaxation, while did both human CGRP8-37 (10(-5) M) and neurokinin-1 receptor antagonists (spantide, 2 x 10(-5) M and FK888, 3 x 10(-6) M) in PCV. NG-nitro-L-arginine methyl ester (10(-4) M) did not significantly attenuate either the NANC-or CGRP-induced relaxation in AMA. However, it significantly did attenuate both the NANC-and SP-induced relaxation, and it also considerably attenuated CGRP-induced relaxation although insignificantly, in PCV. Thus, CGRP could be significantly responsible for the NO-independent NANC relaxation in AMA, whereas both CGRP and SP could additionally relax PCV in a NO-dependent manner.
H Matsuda; F Ohmori; M Uematsu; S Nagata; K Miyatake
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the autonomic nervous system     Volume:  55     ISSN:  0165-1838     ISO Abbreviation:  J. Auton. Nerv. Syst.     Publication Date:  1995 Oct 
Date Detail:
Created Date:  1996-08-26     Completed Date:  1996-08-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8003419     Medline TA:  J Auton Nerv Syst     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  115-22     Citation Subset:  IM    
Department of Cardiovascular Dynamics, National Cardiovascular Center Research Institute, Osaka, Japan.
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MeSH Terms
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Arginine / analogs & derivatives,  pharmacology
Calcitonin Gene-Related Peptide / pharmacology
Capsaicin / pharmacology
Dipeptides / pharmacology
Dose-Response Relationship, Drug
Electric Stimulation
Guinea Pigs
Indoles / pharmacology
Muscle Relaxation / drug effects,  physiology*
Muscle, Smooth, Vascular / innervation,  physiology*
NG-Nitroarginine Methyl Ester
Neurons, Afferent / drug effects,  physiology*
Neuropeptides / physiology*
Nitric Oxide / antagonists & inhibitors,  physiology*
Prostaglandin Endoperoxides, Synthetic / pharmacology
Receptors, Neurotransmitter / antagonists & inhibitors
Substance P / analogs & derivatives,  pharmacology
Thromboxane A2 / analogs & derivatives,  pharmacology
Vasoconstrictor Agents / pharmacology
Reg. No./Substance:
0/Dipeptides; 0/Indoles; 0/Neuropeptides; 0/Prostaglandin Endoperoxides, Synthetic; 0/Receptors, Neurotransmitter; 0/Vasoconstrictor Agents; 10102-43-9/Nitric Oxide; 138449-07-7/FK 888; 33507-63-0/Substance P; 404-86-4/Capsaicin; 50903-99-6/NG-Nitroarginine Methyl Ester; 57576-52-0/Thromboxane A2; 74-79-3/Arginine; 76898-47-0/15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; 83652-28-2/Calcitonin Gene-Related Peptide; 91224-37-2/spantide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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