Document Detail

Different metabolic recycling of the lipid components of exogenous sulphatide in human fibroblasts.
MedLine Citation:
PMID:  3415659     Owner:  NLM     Status:  MEDLINE    
Cultured human fibroblasts were fed with two differently labelled sulphatide molecules [one labelled on C-3 of the sphingosine (Sph) moiety [( Sph-3H]sulphatide), the second on C-1 of stearic acid [( stearoyl-14C]sulphatide)], and the intracellular metabolic fate of radioactivity was monitored. Incorporated radioactivity was almost all recovered in the total lipid extract, regardless of the labelling position of the added sulphatide; however, large differences in the level of incorporation occurred among labelled glycosphingolipids. For example, sphingomyelin was present as the major radiolabelled lipid after [Sph-3H]-sulphatide incubation, but was detectable only in trace amounts after [stearoyl-14C]sulphatide administration; in the latter case the radioactivity was located predominantly in glycerophospholipids. From this finding it can be inferred that the free long-chain base (sphingosine) that originates from lysosomal catabolism of sulphatide is mainly, and quite specifically, utilized for sphingomyelin biosynthesis, whereas the ceramide moiety is not; conversely the fatty acid released from ceramide is non-specifically re-utilized for phospholipid biosynthesis.
M Trinchera; U Wiesmann; M Pitto; D Acquotti; R Ghidoni
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  252     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1988 Jun 
Date Detail:
Created Date:  1988-09-28     Completed Date:  1988-09-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  375-9     Citation Subset:  IM    
Department of Medical Chemistry and Biochemistry, Medical School, University of Milan, Italy.
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MeSH Terms
Carbon Radioisotopes / diagnostic use
Cells, Cultured
Chromatography, Thin Layer
Fibroblasts / metabolism*
Lipid Metabolism*
Phospholipids / biosynthesis
Sphingomyelins / biosynthesis
Sphingosine / metabolism
Stearic Acids / metabolism
Sulfoglycosphingolipids / metabolism*
Tritium / diagnostic use
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Phospholipids; 0/Sphingomyelins; 0/Stearic Acids; 0/Sulfoglycosphingolipids; 10028-17-8/Tritium; 123-78-4/Sphingosine; 57-11-4/stearic acid

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