| Different mechanisms of soy isoflavones in cell cycle regulation and inhibition of invasion. | |
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MedLine Citation:
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PMID: 12552999 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Soy isoflavones, genistein, daidzein and glycitein, are thought to have beneficial effects on cancer prevention. MATERIALS AND METHODS: We used cell cycle analysis, invasion assay and immunoblotting to determine the effects of genistein and glycitein on Jurkat T cells. RESULTS: Glycitein inhibited Jurkat cell invasion at a level comparable to the inhibition by genistein. Both genistein and glycitein down-regulated MMP-13 proteolytic activity by 60-70% and MMP-8 expression. Caffeine could block G2/M arrest by genistein, but was unable to block the inhibition of invasion by genistein and glycitein. We also demonstrated that glycitein inhibited proteintyrosine phosphorylation in Jurkat cells. CONCLUSION: We determined, for the first time, that glycitein inhibited Jurkat cell invasion, in part through the down-regulation of MMP-13 activity and MMP-8 expression. Our findings also suggest that soy isoflavones may utilize different mechanisms to exert their effects on cell cycle progression and invasiveness of Jurkat cells. |
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Authors:
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Myoung H Kim; Angelica M Gutierrez; Ronald H Goldfarb |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 22 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 2002 Nov-Dec |
Date Detail:
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Created Date: 2003-01-29 Completed Date: 2003-03-07 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 3811-7 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology and Immunology and Institute for Cancer Research, University of North Texas Health Science Center, Fort Worth, TX 76107-2699, USA. mkim@hsc.unt.edu |
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals Anticarcinogenic Agents / pharmacology* Caffeine / pharmacology Cell Cycle / drug effects*, physiology Collagenases / metabolism Genistein / pharmacology* Humans Isoflavones / pharmacology* Jurkat Cells / cytology, drug effects*, enzymology Matrix Metalloproteinase 13 Matrix Metalloproteinase 8 / metabolism Mice Neoplasm Invasiveness Phosphorylation / drug effects Protein-Tyrosine Kinases / antagonists & inhibitors, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Anticarcinogenic Agents; 0/Isoflavones; 40957-83-3/glycitein; 446-72-0/Genistein; 58-08-2/Caffeine; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 3.4.24.-/Collagenases; EC 3.4.24.-/MMP13 protein, human; EC 3.4.24.-/Matrix Metalloproteinase 13; EC 3.4.24.-/Mmp13 protein, mouse; EC 3.4.24.34/Matrix Metalloproteinase 8 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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