Document Detail

Different involvement of radical species in irradiated and bystander cells.
MedLine Citation:
PMID:  18979315     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To examine whether nitric oxide (NO) and other radical species are involved in radiation-induced bystander effects in normal human fibroblasts. MATERIALS AND METHODS: Bystander effects were modeled by co-culture of non-irradiated cells with X-irradiated cells, and induction levels of micronuclei in co-cultured non-irradiated cells were examined. Three types of radical scavenger, 2-(4-carboxyphenyl)-4,4,5,5- tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO), dimethylsulfoxide (DMSO) and ascorbic acid phosphoric ester magnesium salt (APM), were used to discover which types of radicals are involved in bystander responses. RESULTS: When irradiated cells were treated with c-PTIO, known to be an NO scavenger, the induction of micronuclei in non-irradiated bystander cells was suppressed. On the other hand, bystander effects were most effectively suppressed when non-irradiated bystander cells were treated with ascorbic acid, known to be a scavenger of long lived radicals. CONCLUSION: These results suggest that NO participates in bystander signal formation in irradiated cells but not in bystander cells that are receiving bystander signals.
Tadayuki Harada; Genro Kashino; Keiji Suzuki; Naoki Matsuda; Seiji Kodama; Masami Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of radiation biology     Volume:  84     ISSN:  0955-3002     ISO Abbreviation:  Int. J. Radiat. Biol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-11-03     Completed Date:  2009-01-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8809243     Medline TA:  Int J Radiat Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  809-14     Citation Subset:  IM; S    
Division of Radiation Biology, Department of Radiology and Radiation Biology, Course of Life Sciences and Radiation Research, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan.
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MeSH Terms
Bystander Effect / drug effects,  radiation effects*
Cell Line
Coculture Techniques
Fibroblasts / cytology*,  drug effects,  metabolism,  radiation effects*
Free Radical Scavengers / pharmacology
Free Radicals / metabolism*
Nitric Oxide / metabolism
Reactive Oxygen Species / metabolism
Reg. No./Substance:
0/Free Radical Scavengers; 0/Free Radicals; 0/Reactive Oxygen Species; 10102-43-9/Nitric Oxide

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