Document Detail


Different functions of intestinal and liver-type fatty acid-binding proteins in intestine and in whole body energy homeostasis.
MedLine Citation:
PMID:  21350192     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
It has long been known that mammalian enterocytes coexpress two members of the fatty acid-binding protein (FABP) family, the intestinal FABP (IFABP) and the liver FABP (LFABP). Both bind long-chain fatty acids and have similar though not identical distributions in the intestinal tract. While a number of in vitro properties suggest the potential for different functions, the underlying reasons for expression of both proteins in the same cells are not known. Utilizing mice genetically lacking either IFABP or LFABP, we directly demonstrate that each of the enterocyte FABPs participates in specific pathways of intestinal lipid metabolism. In particular, LFABP appears to target fatty acids toward oxidative pathways and dietary monoacylglycerols toward anabolic pathways, while IFABP targets dietary fatty acids toward triacylglycerol synthesis. The two FABP-null models also displayed differences in whole body response to fasting, with LFABP-null animals losing less fat-free mass and IFABP-null animals losing more fat mass relative to wild-type mice. The metabolic changes observed in both null models appear to occur by nontranscriptional mechanisms, supporting the hypothesis that the enterocyte FABPs are specifically trafficking their ligands to their respective metabolic fates.
Authors:
William Stacy Lagakos; Angela Marie Gajda; Luis Agellon; Bert Binas; Victor Choi; Bernadette Mandap; Timothy Russnak; Yin Xiu Zhou; Judith Storch
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-02-24
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  300     ISSN:  1522-1547     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-28     Completed Date:  2011-06-28     Revised Date:  2012-05-01    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G803-14     Citation Subset:  IM    
Affiliation:
Department of Nutritional Sciences, Rutgers, the State University of New Jersey, New Brunswick, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Body Composition / genetics,  physiology
Body Weight / genetics,  physiology
Eating / genetics,  physiology
Energy Metabolism / physiology*
Enterocytes / metabolism
Fatty Acid-Binding Proteins / biosynthesis,  genetics,  physiology*
Fatty Acids / metabolism
Feces / chemistry
Gene Expression / physiology
Genotype
Homeostasis / physiology
Intestinal Mucosa / metabolism
Intestines / metabolism*,  physiology*
Lipid Metabolism / genetics,  physiology
Lipids / blood
Liver / metabolism*,  physiology*
Mice
Mice, Inbred C57BL
Mice, Knockout
Oxidation-Reduction
Grant Support
ID/Acronym/Agency:
DK-38389/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acid-Binding Proteins; 0/Fatty Acids; 0/Lipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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