Document Detail

Different fate of sibling cells upon inhibition of transcription in G1.
MedLine Citation:
PMID:  8299729     Owner:  NLM     Status:  MEDLINE    
In normally cycling populations of NIH3T3 cells, both sibling cells generated by a mitotic division require similar periods of time for completion of the cell cycle, with variations (intersibling times) of < or = 3 h for > 90% of the population. In this study, we analyzed by time-lapse video recording the effect of the RNA polymerase II inhibitor alpha-amanitin on the intersibling times of cells exposed to the drug at defined postmitotic ages. Our results led to the identification of three subpopulations of NIH3T3 cells. In the first one, both sibling cells were highly sensitive to alpha-amanitin. These cells were exposed to the inhibitor at a postmitotic age of < or = 2 h. The second subpopulation was composed of cells where one sibling showed normal kinetics of cell cycle progression, while the other sibling had an increased cell cycle length (intersibling times of up to 12 h) or did not divide at all during the period of observation. These cells were 2-8 h old at the time of treatment. In the third subpopulation, representing cells at later stages in the cell cycle, no significant increase in intersibling times was observed. These data indicate that alpha-amanitin increases the intersibling times of cells exposed to the drug during G1. In addition, our results suggest that the variable-length period of G1, thought to be located in late G1, is dependent on RNA polymerase II transcription.
S Adolph; R Müller
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  210     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-03-10     Completed Date:  1994-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  349-52     Citation Subset:  IM    
Institut für Molekularbiologie und Tumorforschung (IMT), Philipps-Universität Marburg, Germany.
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MeSH Terms
3T3 Cells
Amanitins / toxicity*
Cell Cycle / drug effects,  physiology*
G1 Phase / physiology*
RNA Polymerase II / antagonists & inhibitors
Transcription, Genetic / drug effects*
Video Recording
Reg. No./Substance:
0/Amanitins; EC 2.7.7.-/RNA Polymerase II

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