Document Detail


Different effects of prostaglandins E2 and F2 alpha on triglyceride levels in uterine smooth muscle from diabetic diestrous rats.
MedLine Citation:
PMID:  3865236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Triglyceride (TG) concentrations in uterine strips isolated from diestrous normal rats, from diestrous streptozotocin (single iv. injection of 65 mg X Kg body weight-1)-diabetic rats and from diestrous diabetic animals, treated in vivo with insulin (protamine zinc insulin 4U X day-1, 6 days, sc.), were measured. Determinations were made immediately following killing (0 min, or postisolation) as well as after an incubation period in glucose-free medium (60 min, or postincubation), with or without additions. The basal or postisolation levels of TGs (0 min) in the uterus from control normal rats (TGs: 17.0 +/- 0.5 mumol X g-1, d.w.; blood glucose: 100.4 +/- 4.8 mg X dl-1) were lower (P less than 0.001) than in streptozotocin-diabetics (TGs: 28.5 +/- 0.6 mumol X g-1, d.w.; blood glucose: 302.0 +/- 10.1 mg X dl-1) and the treatment with insulin (blood glucose: 165.0 +/- 14.7 mg X dl-1), restored TGs towards normal (19.3 +/- 1.3 mumol X g-1, d.w.). The postisolation levels of TGs (0 min) in the uterus from normal controls did not differ in comparison with postincubation values (60 min), either without additions or in presence of indomethacin (5 X 10(-6)M) or of prostaglandins (PGs) E2 or F2 alpha, at 10(-9)M. On the contrary, in uteri from diabetic animals, the higher initial TG levels diminished spontaneously in comparison to controls (P less than 0.001) following 60 min of incubation without additions; this decrement being antagonized by indomethacin or by propranolol (10(-6)M). Moreover, exogenous PGE2 failed to alter the effect of indomethacin, whereas PGF2 alpha evoked its abolition. In addition, propranolol, which effectively blocked the spontaneous decrement of TGs as did indomethacin, prevented also the influence of PGF2 alpha on the action of the cyclo-oxygenase inhibitor. In the case of uteri from diabetic rats treated in vivo with insulin, TG levels remained unaltered at the end of an incubating period of 60 min without additions and so they did following the administration of indomethacin or PGF2 alpha. The results document that the regulation of TGs in the diestrous rat uteri, suspended in glucose-free solution, is affected differently by PGE2 and F2 alpha, depending on the existence or not of an untreated diabetic condition. They also suggest that the action of PGF2 alpha may involve the activation of tissue beta-adrenoreceptors.
Authors:
E K Macciocchi; J A Linares; A Goldraij; M F Gimeno; A L Gimeno
Related Documents :
21522126 - Brown adipocyte progenitor population is modified in obese and diabetic skeletal muscle.
9915626 - Sympathetic hyperactivity in wistar rats with insulin-resistance.
22102916 - Substrate-favored lysosomal and proteasomal pathways participate in the normal balance ...
22095236 - Elevated levels of renal and circulating nop-7-associated 2 (nsa2) in rat and mouse mod...
10877006 - Mealtime glucose regulation by nateglinide in type-2 diabetes mellitus.
15249606 - High lipoprotein (a), diabetes, and the extent of symptomatic intracranial atherosclero...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Prostaglandins, leukotrienes, and medicine     Volume:  20     ISSN:  0262-1746     ISO Abbreviation:  Prostaglandins Leukot Med     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1986-01-21     Completed Date:  1986-01-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8206868     Medline TA:  Prostaglandins Leukot Med     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  99-109     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Diabetes Mellitus, Experimental / metabolism*
Diestrus / drug effects*
Dinoprost
Dinoprostone
Estrus / drug effects*
Female
Indomethacin / pharmacology
Kinetics
Muscle, Smooth / drug effects,  metabolism*
Propranolol / pharmacology
Prostaglandins E / pharmacology*
Prostaglandins F / pharmacology*
Rats
Rats, Inbred Strains
Triglycerides / metabolism*
Uterus / drug effects,  metabolism*
Chemical
Reg. No./Substance:
0/Prostaglandins E; 0/Prostaglandins F; 0/Triglycerides; 363-24-6/Dinoprostone; 525-66-6/Propranolol; 53-86-1/Indomethacin; 551-11-1/Dinoprost

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Pulmonary vascular response to high-frequency oscillatory ventilation in dogs: lack of effect of ind...
Next Document:  The role of prostaglandins in aldosterone and corticosterone secretion by isolated perfused rat zona...