Document Detail


Different effects of paraquat on microsomal lipid peroxidation in mouse brain, lung and liver.
MedLine Citation:
PMID:  1907736     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Paraquat stimulates NADPH-Fe(2+)-dependent microsomal lipid peroxidation in mouse brain and strongly inhibits it in the liver. In lung microsomes, the lipid peroxidation was stimulated by paraquat at 10(-4) M, but not at higher doses. An antioxidant action of paraquat seemed to account, at least in part, for the lack of stimulation in lung microsomes, but it was inappropriate to explain the result in hepatic microsomes. There was no apparent correlation between the effects of paraquat on the lipid peroxidation and on the activity of NADPH-cytochrome P-450 reductase, the enzyme which initiates redox cycling of paraquat, resulting in generation of active oxygen species. In fact, the effect of paraquat on the lipid peroxidation was independent of paraquat radical production, an intermediate in the cycle. However, the inhibitory potency of N-ethylmaleimide on NADPH-cytochrome P-450 reductase activity paralleled that on the lipid peroxidation stimulated by paraquat in brain and lung. These findings indicate that the effect of paraquat on microsomal lipid peroxidation differs among the organs and that other factors, besides NADPH-cytochrome P-450 reductase, might be involved in the stimulation of lipid peroxidation by paraquat.
Authors:
S Hara; T Endo; F Kuriiwa; S Kano
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Pharmacology & toxicology     Volume:  68     ISSN:  0901-9928     ISO Abbreviation:  Pharmacol. Toxicol.     Publication Date:  1991 Apr 
Date Detail:
Created Date:  1991-09-10     Completed Date:  1991-09-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8702180     Medline TA:  Pharmacol Toxicol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  260-5     Citation Subset:  IM    
Affiliation:
Department of Forensic Medicine, Tokyo Medical College, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / drug effects*,  metabolism
Ethylmaleimide / pharmacology
Free Radicals
Lipid Peroxidation / drug effects*
Lung / drug effects*,  metabolism
Male
Mice
Mice, Inbred ICR
Microsomes / drug effects*,  metabolism
Microsomes, Liver / drug effects*,  metabolism
NADPH-Ferrihemoprotein Reductase / drug effects,  metabolism
Paraquat / pharmacology*
Chemical
Reg. No./Substance:
0/Free Radicals; 128-53-0/Ethylmaleimide; 4685-14-7/Paraquat; EC 1.6.2.4/NADPH-Ferrihemoprotein Reductase

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