| Different effects of p58PITSLRE on the apoptosis induced by etoposide, cycloheximide and serum-withdrawal in human hepatocarcinoma cells. | |
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MedLine Citation:
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PMID: 12349909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Minimal overexpression of the p58PITSLRE protein kinase in Chinese hamster ovary cells induces telephase delay, abnormal cytokinesis, retarded cell growth and apoptosis. Fas mediated T cell death is correlated with p58PITSLRE proteolysis and an increase in its histone H1 kinase activity. In this study, it was found that p58PITSLRE had different effects on the apoptosis induced by etoposide, cycloheximide and serum-withdrawal in human hepatocarcinoma cells. The ectopic expression of p58PITSLRE in human hepatocarcinoma cells suppressed apoptosis induced by etoposide, while enhancing the apoptosis induced by cycloheximide and serum-withdrawal respectively. Elevated expression of p58PITSLRE was found during the apoptosis induced by etoposide, whereas most of p58PITSLRE was proteolytically processed during apoptosis induced by cycloheximide and serum-withdrawal. Furthermore, transient transfection of p50PITSLRE resembling the proteolytic form of p58PITSLRE enhanced the 7,721 cells susceptibility to apoptosis induced by all the three stimuli. These findings suggest that the full-length p58PITSLRE might protect the cells from the apoptosis induced by etoposide and its proteolysis might contribute to and enhance the apoptosis induced by cycloheximide and serum-withdrawal respectively. |
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Authors:
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Ming M Cai; Song W Zhang; Si Zhang; She Chen; Jun Yan; Xiao Y Zhu; Yun Hu; Chun Chen; Jian X Gu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 238 ISSN: 0300-8177 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2002 Sep |
Date Detail:
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Created Date: 2002-09-27 Completed Date: 2003-09-29 Revised Date: 2011-10-10 |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 49-55 Citation Subset: IM |
Affiliation:
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Gene Research Center, Medical Center of Fudan University, Shanghai, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects* Blood Volume / drug effects Cell Death / drug effects Chemiluminescent Measurements Culture Media, Serum-Free / pharmacology* Cyclin-Dependent Kinases Cycloheximide / pharmacology* Etoposide / pharmacology* Female Humans In Situ Nick-End Labeling Liver / drug effects, metabolism, pathology Liver Neoplasms / metabolism, pathology Protein Kinases / genetics, metabolism* Protein-Serine-Threonine Kinases Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Serum-Free; 33419-42-0/Etoposide; 66-81-9/Cycloheximide; EC 2.7.-/Protein Kinases; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.22/CDK11a protein, human; EC 2.7.11.22/Cyclin-Dependent Kinases |
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