Document Detail

Different effects of carbon tetrachloride on carcinogen-induced hepatocellular carcinoma and normal liver of the rat: lowered lipid peroxidation and accelerated necrosis in cancer.
MedLine Citation:
PMID:  1905697     Owner:  NLM     Status:  MEDLINE    
To investigate molecular responses to lipid peroxidative stimuli in neoplastic cells, lipid peroxidation was induced in liver of rats bearing 3'-methyl-4-dimethylaminoazobenzene-induced hepatocellular carcinoma by injecting a high dose of carbon tetrachloride (CCl4), a strong lipoperoxidative reagent. Normal rat livers with or without CCl4 treatment served as controls. CCl4 administration markedly provoked fatty metamorphosis, visualized by oil red O staining, in normal livers while minimal fatty changes were seen in hepatocellular carcinomas, where necrosis was often observed instead. After CCl4 treatment, the thiobarbituric acid values (representing levels of lipid peroxides in the tissue) were increased two-fold in the untreated normal liver, but were unchanged in the cancer tissue. Levels of vitamin C, an acutely reactive antioxidant, measured by high-performance liquid chromatography were not influenced by the CCl4 injection in the cancer tissue whereas a significant decrease was evident in normal livers. The total fatty acid content, measured by gas chromatography, was significantly lower in the cancer tissue than in the normal liver while the ratio of polyunsaturated fatty acids (PUFAs) in total fatty acids was little changed. Resistance of hepatocellular cancer cells to fatty metamorphosis and their susceptibility to necrosis induced by free radicals may be due to the paucity of the target PUFAs in their cell membrane fraction, resulting in low levels of lipid peroxides. Peroxidation of PUFAs might act as a "shock absorber" against free radical-induced toxic cell death in normal cells.
N Lertprasertsuke; M Shinoda; S Takekoshi; Y Tsutsumi; Y Yamamoto; E Niki; K Watanabe
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Japanese journal of cancer research : Gann     Volume:  82     ISSN:  0910-5050     ISO Abbreviation:  Jpn. J. Cancer Res.     Publication Date:  1991 May 
Date Detail:
Created Date:  1991-08-02     Completed Date:  1991-08-02     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8509412     Medline TA:  Jpn J Cancer Res     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  503-10     Citation Subset:  IM    
Department of Pathology, Tokai University School of Medicine, Isehara.
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MeSH Terms
Ascorbic Acid / metabolism
Azo Compounds
Carbon Tetrachloride / pharmacology*
Fatty Acids / metabolism
Fatty Liver / chemically induced
Lipid Peroxidation*
Liver / drug effects,  metabolism*,  pathology
Liver Neoplasms, Experimental / chemically induced,  metabolism*,  pathology
Thiobarbiturates / metabolism
Reg. No./Substance:
0/Azo Compounds; 0/Fatty Acids; 0/Thiobarbiturates; 1320-06-5/oil red O; 50-81-7/Ascorbic Acid; 504-17-6/thiobarbituric acid; 55-80-1/Methyldimethylaminoazobenzene; 56-23-5/Carbon Tetrachloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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