| Different effects of arsenate and phosphonoformate on P(i) transport adaptation in opossum kidney cells. | |
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MedLine Citation:
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PMID: 19553564 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The main nonhormonal mechanism for controlling inorganic phosphate (P(i)) homeostasis is renal adaptation of the proximal tubular P(i) transport rate to changes in dietary phosphate content. Opossum kidney (OK) cell line is an in vitro renal model that maintains the ability of renal adaptation to the extracellular P(i) concentration. We have studied how two competitive inhibitors of P(i) transport, arsenate [As(V)] and phosphonoformate (PFA), affect adaptation to low and high P(i) concentrations. OK cells show very high affinity for As(V) (inhibitory constant, K(i) 0.12 mM) when compared with the rat kidney. As(V) very efficiently reversed the adaptation of OK cells to low P(i) (0.1 mM), whereas PFA induced adaptation similar to 0.1 mM P(i). Adaptation with 2 mM P(i) or As(V) was characterized by decreases in the maximal velocity (V(max)) of P(i) transport and an abundance of the NaPi-IIa P(i) transporter in the plasma membrane, shown by the protein biotinylation. Conversely, PFA and 0.1 mM P(i) increased the V(max) and transporter abundance. Changes in the V(max) were limited to a 50% variation, which was not paralleled by changes in the concentration of P(i) or of the inhibitor. OK cells are very sensitive to As(V), but the effects are reversible and noncytotoxic. These effects can be interpreted as As(V) being transported into the cell, thereby mimicking a high P(i) concentration. PFA blocks the uptake of P(i) but is not transported, and it therefore simulates a low P(i) concentration inside the cell. To conclude, a mathematical definition of the adaptation process is reported, thereby explaining the limited changes in P(i) transport V(max). |
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Authors:
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Ricardo Villa-Bellosta; V??ctor Sorribas |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-24 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: 297 ISSN: 1522-1563 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-07 Completed Date: 2009-10-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: C516-25 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Toxicology, Univ. of Zaragoza, Zaragoza, Spain. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arsenates / pharmacology* Cell Line Dose-Response Relationship, Drug Foscarnet / pharmacology* Kidney / cytology*, drug effects* Oocytes Opossums Phosphorus / metabolism* Sodium-Phosphate Cotransporter Proteins, Type IIa / metabolism Xenopus |
| Chemical | |
Reg. No./Substance:
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0/Arsenates; 0/Sodium-Phosphate Cotransporter Proteins, Type IIa; 4428-95-9/Foscarnet; 7723-14-0/Phosphorus |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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