Document Detail

Different effect of ouabain on endothelin-1-induced extracellular signal-regulated kinase stimulation in rat heart and tail artery.
MedLine Citation:
PMID:  12658056     Owner:  NLM     Status:  MEDLINE    
Endogenous ouabain may play a role in the control of cardiovascular system function. In this study, we investigated the effects of a long-term ouabain treatment on basal and endothelin-1 (ET-1)-induced phosphorylation of cardiac and vascular extracellular signal-regulated kinases 1 and 2 (ERK-1 and ERK-2), which are involved in several cardiac and vascular physiologic and pathologic conditions. Our results show that the hearts from ouabain-treated rats have a higher basal level of ERK-1 and ERK-2 phosphorylation compared with untreated rats. Perfusion of the hearts with ET-1 increased ERK-1 and ERK-2 phosphorylation both in ouabain-treated and in control rats, with a larger stimulatory effect in ouabain-treated animals. On the contrary, exposure of endothelium-free tail artery to ET-1 increased ERKs phosphorylation both in treated and untreated rats, but this effect was blunted in ouabain-treated rats. These findings demonstrate that ouabain treatment has opposite effects on basal and ET-1-induced ERKs phosphorylation in the heart and in the tail artery of the rat.
Giulio Ceolotto; Michelangelo Sartori; Italia Papparella; Marco Candiotto; Elisabetta Baritono; Amelia Filippelli; Gabriella Cargnelli; Sisto Luciani; Andrea Semplicini; Sergio Bova
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  41     ISSN:  0160-2446     ISO Abbreviation:  J. Cardiovasc. Pharmacol.     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-03-26     Completed Date:  2003-10-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  553-61     Citation Subset:  IM    
Departments of Clinical and Experimental Medicine, University of Padua, Padua, Italy.
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MeSH Terms
Arteries / drug effects*,  enzymology,  physiology*
Coronary Vessels / drug effects*,  enzymology,  physiology*
Endothelin-1 / pharmacology*
Mitogen-Activated Protein Kinases / biosynthesis*
Ouabain / pharmacology*
Rats, Sprague-Dawley
Tail / blood supply*
Reg. No./Substance:
0/Endothelin-1; 630-60-4/Ouabain; EC Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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