Document Detail


Different domains of C. elegans PAR-3 are required at different times in development.
MedLine Citation:
PMID:  20678977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polarity is a fundamental cellular feature that is critical for generating cell diversity and maintaining organ functions during development. In C. elegans, the one-cell embryo is polarized via asymmetric localization of the PAR proteins, which in turn are required to establish the future anterior-posterior axis of the embryo. PAR-3, a conserved PDZ domain-containing protein, acts with PAR-6 and PKC-3 (atypical protein kinase; aPKC) to regulate cell polarity and junction formation in a variety of cell types. To understand how PAR-3 localizes and functions during C. elegans development, we produced targeted mutations and deletions of conserved domains of PAR-3 and examined the localization and function of the GFP-tagged proteins in C. elegans embryos and larvae. We find that CR1, the PAR-3 self-oligomerization domain, is required for PAR-3 cortical distribution and function only during early embryogenesis and that PDZ2 is required for PAR-3 to accumulate stably at the cell periphery in early embryos and at the apical surface in pharyngeal and intestinal epithelial cells. We also show that phosphorylation at S863 by PKC-3 is not essential in early embryogenesis, but is important in later development. Surprisingly neither PDZ1 nor PDZ3 are essential for localization or function. Our results indicate that the different domains and phosphorylated forms of PAR-3 can have different roles during C. elegans development.
Authors:
Bingsi Li; Heon Kim; Melissa Beers; Kenneth Kemphues
Related Documents :
11752627 - Photoactivated gene expression for cell fate mapping and cell manipulation.
10929107 - Cell division events are essential for embryo patterning and morphogenesis: studies on ...
17186547 - Beneficial effects of dithiothreitol on relative levels of glutathione s-transferase ac...
18267947 - Regulation of cell cycle activity in the embryo of barley seeds during germination as r...
10385917 - Effects of fluorescent light on growth of bovine retinal pigment epithelial cells in vi...
9766887 - Flow cytometric detection of ebv (eber snrna) using peptide nucleic acid probes.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-06-04
Journal Detail:
Title:  Developmental biology     Volume:  344     ISSN:  1095-564X     ISO Abbreviation:  Dev. Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-03     Completed Date:  2010-08-30     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  745-57     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Caenorhabditis elegans / genetics,  metabolism*,  physiology
Cell Polarity / genetics,  physiology*
Epithelial Cells / cytology,  metabolism,  physiology
Membrane Proteins
Neoplasm Proteins
PDZ Domains
Poly(ADP-ribose) Polymerases / genetics,  metabolism
Protein Kinase C / genetics,  metabolism*,  physiology
Proteins / genetics,  metabolism
Transcription Factors / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
GM079112/GM/NIGMS NIH HHS; HD27689/HD/NICHD NIH HHS; R01 GM079112/GM/NIGMS NIH HHS; R01 GM079112-19/GM/NIGMS NIH HHS; R01 HD027689/HD/NICHD NIH HHS; R01 HD027689-15/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/JTB protein, human; 0/Membrane Proteins; 0/Neoplasm Proteins; 0/Proteins; 0/Transcription Factors; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 2.7.11.13/Protein Kinase C
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Corticoperiosteal flap in the treatment of nonunions and small bone gaps: Technical details and expa...
Next Document:  Induction of human keratinocytes into enamel-secreting ameloblasts.