Document Detail

Different activities of the reovirus FAST proteins and influenza hemagglutinin in cell-cell fusion assays and in response to membrane curvature agents.
MedLine Citation:
PMID:  19931884     Owner:  NLM     Status:  MEDLINE    
The reovirus fusion-associated small transmembrane (FAST) proteins evolved to induce cell-cell, rather than virus-cell, membrane fusion. It is unclear whether the FAST protein fusion reaction proceeds in the same manner as the enveloped virus fusion proteins. We now show that fluorescence-based cell-cell and cell-RBC hemifusion assays are unsuited for detecting lipid mixing in the absence of content mixing during FAST protein-mediated membrane fusion. Furthermore, membrane curvature agents that inhibit hemifusion or promote pore formation mediated by influenza hemagglutinin had no effect on p14-induced cell-cell fusion, even under conditions of limiting p14 concentrations. Standard assays used to detect fusion intermediates induced by enveloped virus fusion proteins are therefore not applicable to the FAST proteins. These results suggest the possibility that the nature of the fusion intermediates or the mechanisms used to transit through the various stages of the fusion reaction may differ between these distinct classes of viral fusogens.
Eileen K Clancy; Chris Barry; Marta Ciechonska; Roy Duncan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-20
Journal Detail:
Title:  Virology     Volume:  397     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-02-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  119-29     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Department of Microbiology and Immunology, Dalhousie University, Halifax, Nova Scotia, Canada B3H 1X5.
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MeSH Terms
Cell Fusion
Cell Membrane / drug effects
Membrane Proteins / metabolism*
Reoviridae / pathogenicity*,  physiology*
Viral Proteins / metabolism*
Virus Internalization*
Grant Support
//Canadian Institutes of Health Research
Reg. No./Substance:
0/Membrane Proteins; 0/Viral Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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