Document Detail


Different O-glycosylation of respiratory mucin glycopeptides from a patient with cystic fibrosis.
MedLine Citation:
PMID:  9870359     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The O-linked oligosaccharides from three fractions of highly glycosylated mucin glycopeptides obtained from sputum of a patient with cystic fibrosis were characterized and compared regarding size, composition, sequence and when possible linkage positions. Neutral and sialic acid-containing glycans were permethylated and analyzed by high-temperature GC-MS and MALDI-MS, showing more than 60 different oligosaccharides with a size of up to 15 monosaccharide units. Some of the observed oligosaccharides are novel for respiratory secretions, one being a trifucosylated heptasaccharide with the proposed structure: Fuc-Gal-4(Fuc-3)GlcNAc-(Fuc-)Gal-3GalNAcol. The glycosylation of two of the glycopeptide fractions was similar with regard to the neutral and sialylated oligosaccharides despite their different origins from the sol or gel phase. Analysis of the sulfated oligosaccharides by FAB-MS/MS indicated that the gel fraction contained C-6 linked sulfate groups while the two sol fractions also contained C-3 linked sulfate. The results suggest the presence of different glycosylated mucin domains, probably originating from different mucin glycoforms and/or apoproteins in the airway of cystic fibrosis patients.
Authors:
K A Thomsson; I Carlstedt; N G Karlsson; H Karlsson; G C Hansson
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycoconjugate journal     Volume:  15     ISSN:  0282-0080     ISO Abbreviation:  Glycoconj. J.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1999-03-18     Completed Date:  1999-03-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  823-33     Citation Subset:  IM    
Affiliation:
Department of Medical Biochemistry, Göteborg University, Gothenburg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Cystic Fibrosis*
Female
Gas Chromatography-Mass Spectrometry
Glycopeptides / chemistry*
Glycosylation
Humans
Molecular Sequence Data
Mucins / chemistry*
Oligosaccharides / chemistry
Sequence Analysis
Spectrometry, Mass, Fast Atom Bombardment
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sputum / chemistry*
Sulfuric Acid Esters
Chemical
Reg. No./Substance:
0/Glycopeptides; 0/Mucins; 0/Oligosaccharides; 0/Sulfuric Acid Esters; 0/sialooligosaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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