| Different Disappearance Rates of Plasma Nitrite (NO(2)(-)) Contribute to Apparent Steady-State Arterio-Venous Differences in Anesthetized Animals. | |
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MedLine Citation:
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PMID: 21467641 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A possible cause of arterio-venous (A-V) differences in plasma nitrite (NO(2)(-)) levels under steady-state conditions and kinetic features of NO(2)(-) in arterial and venous blood were examined. In isolated rabbit blood, plasma NO(2)(-) in venous blood disappeared faster than that in arterial blood and was accompanied by a concomitant increase in nitrate (NO(3)(-)), implicating oxidation as the main pertinent metabolic pathway. When data were corrected with respective elimination constants and time durations before plasma separation, no A-V difference was estimated under steady-state. Even after these corrections for NO(2)(-) loading in anesthetized rabbits, a large A-V difference in NO(2)(-) levels (arterial venous) was observed, followed by an exponential decrease in NO(2)(-) levels without a reciprocal increase in NO(3)(-) levels. There was a marked difference in NO(2)(-) decay between in vivo and ex vivo experiments, but no increases in the circulating blood were detected for other substances derived from NO(2)(-), such as methemoglobin or low- and high-molecular weight nitrosothiols. In rats and guinea pigs, absence and presence of the A-V difference were detected under steady-state conditions and after NO(2)(-) loading, respectively. These observations indicate that apparent A-V differences under steady-state are artifacts arising from different rates of NO(2)(-) disappearance in arterial versus venous plasma during sample handling, and that tissue compartments may contribute to changes in NO(2)(-) levels in circulating blood. Therefore, caution is required when evaluating plasma NO(2)(-) levels, especially in venous blood. |
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Authors:
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Takaharu Ishibashi; Naoki Nishizawa; Mihoko Nakamoto-Nomura; Fusae Abe; He Liu; Junko Yoshida; Tomie Kawada; Matomo Nishio |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biological & pharmaceutical bulletin Volume: 34 ISSN: 1347-5215 ISO Abbreviation: Biol. Pharm. Bull. Publication Date: 2011 |
Date Detail:
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Created Date: 2011-04-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9311984 Medline TA: Biol Pharm Bull Country: Japan |
Other Details:
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Languages: eng Pagination: 528-37 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, School of Nursing, Kanazawa Medical University. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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