Document Detail

Differences in the in vitro and in vivo 5-hydroxytryptamine extraction performance among three common microdialysis membranes.
MedLine Citation:
PMID:  1383427     Owner:  NLM     Status:  MEDLINE    
The present study summarizes the results of an in vitro and in vivo comparison of the apparent 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid, and 3,4-dihydroxyphenylacetic acid dialysis performance of three types of membrane frequently used in intracerebral microdialysis experiments. The dialysis fiber types examined were a regenerated cellulose Cuprophan (GF), a proprietary polycarbonate ether (CMA), and a polyacrylonitrile/sodium methallylsulfonate copolymer (HOSPAL). The experiments unexpectedly revealed that the HOSPAL membrane-equipped probes displayed clearly aberrant 5-HT diffusion dynamics compared with GF and CMA probes, demonstrable not only in vitro, but also in in vivo experiments. In vitro, the GF and CMA membrane-equipped probes exhibited maximum relative recovery for 5-HT already in the first 20-min sample, whereas the 5-HT recovery of HOSPAL probes increased in a very slow and protracted manner over a period of a little less than 2 h. The GF and CMA probes further displayed an immediate washout of 5-HT when the probes were subsequently transferred to artificial CSF only-containing medium (no 5-HT), whereas approximately 2 h was required to yield near-total extinction of dialysate 5-HT with the standard HOSPAL probes. In vivo, the rat ventral hippocampal dialysate 5-HT output responses to K+ (100 mM) infusion, to Ca2+ omission, and to systemic 8-hydroxy-2-(di-n-propylamino)tetralin injection were all markedly retarded and blunted when HOSPAL instead of GF membrane-equipped probes were used. However, the 5-hydroxyindoleacetic acid and 3,4-dihydroxyphenylacetic acid extraction in vitro and in vivo were comparable using either of the membrane types.(ABSTRACT TRUNCATED AT 250 WORDS)
R Tao; S Hjorth
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of neurochemistry     Volume:  59     ISSN:  0022-3042     ISO Abbreviation:  J. Neurochem.     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1992-11-20     Completed Date:  1992-11-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985190R     Medline TA:  J Neurochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1778-85     Citation Subset:  IM    
Department of Pharmacology, University of Göteborg, Sweden.
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MeSH Terms
3,4-Dihydroxyphenylacetic Acid / analysis
8-Hydroxy-2-(di-n-propylamino)tetralin / analogs & derivatives,  pharmacology
Cellulose / analogs & derivatives,  chemistry
Dialysis / instrumentation*,  methods
Hippocampus / chemistry*
Hydroxyindoleacetic Acid / analysis
Membranes, Artificial*
Osmolar Concentration
Polymers / chemistry
Potassium / pharmacology
Rats, Sprague-Dawley
Serotonin / analysis*
Reg. No./Substance:
0/Membranes, Artificial; 0/Polymers; 102-32-9/3,4-Dihydroxyphenylacetic Acid; 50-67-9/Serotonin; 54-16-0/Hydroxyindoleacetic Acid; 68593-96-4/5-hydroxy-2-N,N-dipropylaminotetralin; 7440-09-7/Potassium; 78950-78-4/8-Hydroxy-2-(di-n-propylamino)tetralin; 9004-34-6/Cellulose; 9050-09-3/cuprammonium cellulose

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