Document Detail


Differences in transport of fatty acids and expression of fatty acid transporting proteins in adipose tissue of obese black and white women.
MedLine Citation:
PMID:  16339926     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have reported that the rate of de novo triglyceride (TG) synthesis by omental, but not subcutaneous, adipose tissue was higher in African-American women (AAW) than in Caucasian women (CAW). The purpose of this study was to explore the potential mechanisms underlying this increase. Toward that end, we determined the activities of key enzymes in the pathway of TG synthesis, the rates of uptake of fatty acids by adipocytes, mRNA and protein levels of the fatty acid-transporting proteins FAT/CD36 and FATP, and mRNA and protein levels of PPARgamma in omental fat of AAW and CAW. The results showed 1) no difference in the activity of phosphofructokinase, glycerol-3-phosphate dehydrogenase, or diacylglycerol acyltransferase; 2) a higher rate of fatty acid uptake by adipocytes of the AAW; 3) an increase in the mRNA and protein levels of CD36 and FATP4 in the fat of the AAW; and 4) an increase in the mRNA and protein levels of PPARgamma, which can stimulate the expression of CD36 and FATP. These results suggest that the increase in the transport of fatty acid, which is mediated by the overexpression of the transport proteins in the omental adipose tissue of the AAW, might contribute to the higher prevalence of obesity in AAW.
Authors:
Joseph F Bower; Julianne M Davis; Enhui Hao; Hisham A Barakat
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  290     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-12     Completed Date:  2006-02-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E87-E91     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / metabolism
Adipose Tissue / enzymology,  metabolism*
Adult
African Americans*
Antigens, CD36 / genetics,  metabolism
Diacylglycerol O-Acyltransferase / metabolism
European Continental Ancestry Group*
Fatty Acid Transport Proteins / genetics,  metabolism*
Fatty Acids / metabolism*
Female
Gene Expression / genetics
Glycerol-3-Phosphate Dehydrogenase (NAD+) / metabolism
Humans
Obesity, Morbid / enzymology,  metabolism*,  therapy
Oleic Acid / metabolism
Omentum / enzymology,  metabolism
PPAR gamma / genetics,  metabolism
Phosphofructokinases / metabolism
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Subcutaneous Fat / enzymology,  metabolism
United States
Chemical
Reg. No./Substance:
0/Antigens, CD36; 0/Fatty Acid Transport Proteins; 0/Fatty Acids; 0/PPAR gamma; 0/RNA, Messenger; 0/SLC27A4 protein, human; 112-80-1/Oleic Acid; EC 1.1.1.8/Glycerol-3-Phosphate Dehydrogenase (NAD+); EC 2.3.1.20/Diacylglycerol O-Acyltransferase; EC 2.7.1 -/Phosphofructokinases

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