Document Detail


Differences in surface sialic acid and galactosyl residues of two autologous human melanoma cell lines.
MedLine Citation:
PMID:  6544896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell surface carbohydrate differences were observed between two human cell lines initiated from primary melanoma and metastasis of the same patient. Although total sialic acid content was similar in both cell lines, neuraminidase-released sialic acid was twice as high in metastatic cells than that of primary cells. One class of Concanavalin A binding sites with similar affinity constant was found in untreated and neuraminidase-treated cells in both cell lines. Before surface sialic acid release, the primary cell line expressed two classes of Ricinus lectin binding sites with high and low affinity; the cell line of metastatic origin had only one class of Ricinus lectin binding sites with low affinity. After neuraminidase treatment, the number of Ricinus lectin binding sites with low affinity increased two- or three-fold in both cell lines, whereas the high-affinity binding sites were not observed in primary cells. The present data indicated that differences in surface sialic acid level modified the Ricinus lectin binding in two human melanoma cell lines. However, the ability of the cells to bind Concanavalin A was not changed.
Authors:
M Reynier; M Aubery; S Lebec; M Vernay; C Aubert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medical oncology and tumor pharmacotherapy     Volume:  1     ISSN:  0736-0118     ISO Abbreviation:  Med Oncol Tumor Pharmacother     Publication Date:  1984  
Date Detail:
Created Date:  1986-01-08     Completed Date:  1986-01-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8405039     Medline TA:  Med Oncol Tumor Pharmacother     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  157-61     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Binding Sites
Cell Line
Concanavalin A / metabolism
Galactose / analysis*
Humans
Lectins / metabolism
Male
Melanoma / analysis*
Neuraminidase / pharmacology
Plant Lectins*
Sialic Acids / analysis*
Chemical
Reg. No./Substance:
0/Lectins; 0/Plant Lectins; 0/Ricinus communis agglutinin-1; 0/Sialic Acids; 11028-71-0/Concanavalin A; 26566-61-0/Galactose; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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