| Differences in stimulus induced calcium increases in lacrimal gland acinar cells from normal and NZB/NZW F1 female mice. | |
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MedLine Citation:
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PMID: 12658559 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To compare the changes in intracellular free calcium in response to both cholinergic and adrenergic agonists in cells isolated from exorbital lacrimal glands of NZB/NZW F1 female mice (NZB/W) and normal Swiss Webster mice (SW). METHODS: We have loaded cells with Fura-2 and measured total intracellular calcium using ratiomicrofluorometric methods. Isolated cells were also patch-clamped using perforated patch. RESULTS: In all cells, both carbachol (CCh) and phenylephrine (PE) increased intracellular calcium. The calcium increase to both CCh and PE was less in cells from NZB/W animals than in cells from SW animals. In cells from young animals, the baseline calcium levels were the same, but in cells from older (6 months) NZB/W animals, the baseline levels of calcium were 50% higher than those seen in SW animals. CONCLUSION: These data suggest that cells from the NZB/W mice have different calcium dynamics, which could contribute to their compromised fluid secretion. |
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Authors:
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Benjamin Walcott; Gary Matthews; Peter Brink |
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Publication Detail:
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Type: Comparative Study; In Vitro; Journal Article |
Journal Detail:
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Title: Current eye research Volume: 25 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 2002 Oct |
Date Detail:
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Created Date: 2003-03-26 Completed Date: 2003-05-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: England |
Other Details:
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Languages: eng Pagination: 253-60 Citation Subset: IM |
Affiliation:
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Departments of Neurobiology and Behavior, Stony Brook University, New York, Stony Brook 11794-5230, USA. bwalcott@ms.cc.sunysb.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adrenergic alpha-Agonists
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pharmacology* Aging Animals Calcium / metabolism* Carbachol / pharmacology Cholinergic Agonists / pharmacology* Electrophysiology Female Fura-2 / metabolism Lacrimal Apparatus / cytology*, drug effects, metabolism, physiology Membrane Potentials / drug effects Mice Mice, Inbred NZB Patch-Clamp Techniques / methods Phenylephrine / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Adrenergic alpha-Agonists; 0/Cholinergic Agonists; 51-83-2/Carbachol; 59-42-7/Phenylephrine; 7440-70-2/Calcium; 96314-98-6/Fura-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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