Document Detail

Differences in sensitivity of cultured rat brain neuronal and glial cytochrome P450 2E1 to ethanol.
MedLine Citation:
PMID:  16737717     Owner:  NLM     Status:  MEDLINE    
The expression of the cytochrome P450s (CYPs) may vary in the different brain cells depending on their specialization and the presence of different endogenous factors. The present study was initiated to investigate the expression and catalytic activity of the constitutive and inducible forms of CYP2E1, the major ethanol inducible CYP, in cultured rat brain neuronal and glial cells. These cells exhibited relatively two-fold higher activity of N-nitrosodimethylamine demethylase (NDMA-d) when compared with the liver enzyme. Pretreatment with ethanol revealed a significant time and concentration dependent induction in NDMA-d activity in both cell types. Western blot, immunocytochemistry and RT-PCR also indicated significant induction of CYP2E1 in the cultured brain cells. Interestingly, the neuronal cells exhibited greater magnitude of induction than the glial cells. The relatively higher degree of induction in cultures of neurons has indicated enhanced sensitivity of neurons to the inductive effects of ethanol. This enhanced induction of CYP2E1 in neuronal cells has indicated that like regional specificity, cell specificity also exists in the induction of CYP2E1 and other CYPs.
Nidhi Kapoor; Aditya B Pant; Alok Dhawan; Uppendra N Dwievedi; Yogendra K Gupta; Prahlad K Seth; Devendra Parmar
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-05-07
Journal Detail:
Title:  Life sciences     Volume:  79     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-04     Completed Date:  2006-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1514-22     Citation Subset:  IM    
Biochemistry Department, Lucknow University, University Road, Lucknow, India.
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MeSH Terms
Blotting, Western
Brain / drug effects*,  enzymology
Cells, Cultured
Cytochrome P-450 CYP2E1 / analysis,  genetics,  metabolism*
Ethanol / toxicity*
Neuroglia / drug effects*,  enzymology
Neurons / drug effects*,  enzymology
RNA, Messenger / analysis,  metabolism
Reg. No./Substance:
0/RNA, Messenger; 64-17-5/Ethanol; EC P-450 CYP2E1

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