Document Detail


Differences in pH optima and calcium requirements for maturation of the prohormone convertases PC2 and PC3 indicates different intracellular locations for these events.
MedLine Citation:
PMID:  7836407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PC2 and PC3, which is also known as PC1, are subtilisin-like proteases that are involved in the intracellular processing of prohormones and proneuropeptides. Both enzymes are synthesized as propolypeptides that undergo proteolytic maturation within the secretory pathway. An in vitro translation/translocation system from Xenopus egg extracts was used to investigate mechanisms in the maturation of pro-PC3 and pro-PC2. Pro-PC3 underwent rapid (t1/2 < 10 min) processing of the 88-kDa propolypeptide at the sequence RSKR83 to generate the 80-kDa active form of the enzyme. This processing was blocked when the active site aspartate was changed to asparagine, suggesting that an autocatalytic mechanism was involved. In this system, processing of pro-PC3 was optimal between pH 7.0 and 8.0 and was not dependent on additional calcium. These results are consistent with pro-PC3 maturation occurring at an early stage in the secretory pathway, possibly within the endoplasmic reticulum, where the pH would be close to neutral and the calcium concentration less than that observed in later compartments. Processing of pro-PC2 in the Xenopus egg extract was much slower than that of pro-PC3 (t1/2 = 8 h). It exhibited a pH optimum of 5.5-6.0 and was dependent on calcium (K0.5 = 2-4 mM). The enzymatic properties of pro-PC2 processing were similar to that of the mature enzyme. Further studies using mutant pro-PC2 constructs suggested that cleavage of pro-PC2 was catalyzed by the mature 68-kDa PC2 molecule. The results were consistent with pro-PC2 maturation occurring within a late compartment of the secretory pathway that contains a high calcium concentration and low pH.
Authors:
K I Shennan; N A Taylor; J L Jermany; G Matthews; K Docherty
Related Documents :
6677397 - Biochemical factors affecting the fertility of cryopreserved human semen.
7980507 - Protein synthesis is required for the generation of oscillatory current responses in xe...
8793077 - Extracellular calcium negatively modulates tyrosine phosphorylation and tyrosine kinase...
3392517 - Calcium dependence of human sperm fertilizing ability.
1652707 - Renal transplantation and red blood cell sodium transport: role of prednisone treatment.
6446937 - Cytoplasmic and plasma membrane adenosine triphosphatase of polymorphonuclear neutrophi...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  270     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-02-24     Completed Date:  1995-02-24     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1402-7     Citation Subset:  IM    
Affiliation:
Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, United Kingdom.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Aspartic Acid Endopeptidases / genetics,  metabolism*
Base Sequence
Biological Transport
Calcium / metabolism*
Hydrogen-Ion Concentration
Molecular Sequence Data
Oligodeoxyribonucleotides
Proprotein Convertase 2
Proprotein Convertases
Protein Processing, Post-Translational*
RNA, Messenger / genetics,  metabolism
Subtilisins / genetics,  metabolism*
Xenopus
Chemical
Reg. No./Substance:
0/Oligodeoxyribonucleotides; 0/RNA, Messenger; 7440-70-2/Calcium; EC 3.4.-/Proprotein Convertases; EC 3.4.21.-/Subtilisins; EC 3.4.21.94/Proprotein Convertase 2; EC 3.4.23.-/Aspartic Acid Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A Xenopus nonmuscle myosin heavy chain isoform is phosphorylated by cyclin-p34cdc2 kinase during mei...
Next Document:  Human coagulation factor V is activated to the functional cofactor by elastase and cathepsin G expre...