Document Detail


Differences in osteogenic differentiation of adipose-derived stromal cells from murine, canine, and human sources in vitro and in vivo.
MedLine Citation:
PMID:  21788829     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Given the diversity of species from which adipose-derived stromal cells are derived and studied, the authors set out to delineate the differences in the basic cell biology that may exist across species. Briefly, the authors found that significant differences exist with regard to proliferation and osteogenic potentials of adipose-derived stromal cells across species.
METHODS: Adipose-derived stromal cells were derived from human, mouse, and canine sources as previously described. Retinoic acid, insulin-like growth factor-1, and bone morphogenetic protein-2 were added to culture medium; proliferation and osteogenic differentiation were assessed by standardized assays. In vivo methods included seeding 150,000 adipose-derived stromal cells on a biomimetic scaffold and analyzing healing by micro-computed tomography and histology.
RESULTS: Adipose-derived stromal cells from all species had the capability to undergo osteogenic differentiation. Canine adipose-derived stromal cells were the most proliferative, whereas human adipose-derived stromal cells were the most osteogenic (p < 0.05). Human cells, however, had the most significant osteogenic response to osteogenic media. Retinoic acid stimulated osteogenesis in mouse and canine cells but not in human adipose-derived stromal cells. Insulin-like growth factor-1 enhanced osteogenesis across all species, most notably in human- and canine-derived cells.
CONCLUSIONS: Adipose-derived stromal cells derived from human, mouse, and canine all have the capacity to undergo osteogenic differentiation. Canine adipose-derived stromal cells appear to be the most proliferative, whereas human adipose-derived stromal cells appear to be the most osteogenic. Different cytokines and chemicals can be used to modulate this osteogenic response. These results are promising as attempts are made to optimize tissue-engineered bone using adipose-derived stromal cells.
Authors:
Benjamin Levi; Emily R Nelson; Kenneth Brown; Aaron W James; Dan Xu; Robert Dunlevie; Joseph C Wu; Min Lee; Benjamin Wu; George W Commons; Dean Vistnes; Michael T Longaker
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Plastic and reconstructive surgery     Volume:  128     ISSN:  1529-4242     ISO Abbreviation:  Plast. Reconstr. Surg.     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-07-26     Completed Date:  2011-10-19     Revised Date:  2014-10-13    
Medline Journal Info:
Nlm Unique ID:  1306050     Medline TA:  Plast Reconstr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  373-86     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / physiology*,  ultrastructure
Animals
Cell Differentiation / physiology*
Cells, Cultured
Culture Media / chemistry
Dogs
Humans
Mice
Microscopy, Electron, Scanning
Middle Aged
Osteoblasts / cytology*
Osteogenesis / physiology*
Stromal Cells / cytology*
Tissue Engineering / methods
X-Ray Microtomography
Grant Support
ID/Acronym/Agency:
1 R21 DE019274-02/DE/NIDCR NIH HHS; 1 RC2 DE020771-01/DE/NIDCR NIH HHS; 1F32AR057302-01/AR/NIAMS NIH HHS; R01 EB009689/EB/NIBIB NIH HHS; R01EB009689/EB/NIBIB NIH HHS; RC1 HL099117/HL/NHLBI NIH HHS; RC1HL099117/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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