| Differences in the organization and chromosomal allocation of satellite DNA between the European long tailed house mice Mus domesticus and Mus musculus. | |
MedLine Citation:
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PMID: 1971208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We compared the organization of satellite DNA (stDNA) and its chromosomal allocation in Mus domesticus and in Mus musculus. The two stDNAs show similar restriction fragment profiles after digestion (probed with M. domesticus stDNA) with some endonucleases of which restriction sequences are present in the 230-240 bp repetitive unit of the M. domesticus stDNA. In contrast, EcoRI digestion reveals that M. musculus stDNA lacks most of the GAATTC restriction sites, particularly at the level of the half-monomer. The chromosome distribution of stDNA (revealed by an M. domesticus stDNA probe) shows different patterns in the M. domesticus and M. musculus karyotypes, with about 60% of M. domesticus stDNA retained in the M. musculus genome. It is particularly noteworthy that the pericentromeric regions of M. musculus chromosomes 1 and X are totally devoid of M. domesticus stDNA sequences. In both groups, the differences in energy transfer between the stDNA-bound fluorochromes Hoechst 33258 and propidium iodide suggest that AT-rich repeated sequences have a much more clustered array in the M. domesticus stDNA, as if they are organized in tandem repeats longer than those of M. musculus. Considering the data as a whole, it seems likely that the evolutionary paths of the two stDNAs diverged after the generation of the ancestral 230-240 bp stDNA repetitive unit through the amplification, in the M. domesticus genome, of a family repeat which included the EcoRI GAATTC restriction sequence. |
Authors:
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C A Redi; S Garagna; G Della Valle; G Bottiroli; P Dell'Orto; G Viale; F A Peverali; E Raimondi; J Forejt |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Chromosoma Volume: 99 ISSN: 0009-5915 ISO Abbreviation: Chromosoma Publication Date: 1990 Apr |
Date Detail:
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Created Date: 1990-06-27 Completed Date: 1990-06-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985138R Medline TA: Chromosoma Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 11-7 Citation Subset: IM |
Affiliation:
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Dipartimento di Biologia Animale, Universita' di Pavia, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blotting, Southern Chromosome Banding Chromosomes / analysis* DNA, Satellite* / analysis Heterochromatin / analysis Karyotyping Mice / genetics* Muridae / genetics* Polymorphism, Restriction Fragment Length Species Specificity Spectrum Analysis |
| Chemical | |
Reg. No./Substance:
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0/DNA, Satellite; 0/Heterochromatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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