Document Detail

Differences in myosin isoform expression in the subepicardial and subendocardial myocardium during cardiac hypertrophy in the rat.
MedLine Citation:
PMID:  2138524     Owner:  NLM     Status:  MEDLINE    
We have investigated myosin isoform expression during progressive cardiac hypertrophy and the development of congestive heart failure in young male rats. Cardiac enlargement was produced by placing a constricting band (0.024-inch diameter) around the ascending aorta of 25-day-old animals, which resulted in progressively increased stenosis as the rat matured. A 57% and 77% cardiac hypertrophy was observed at 2 and 8 weeks, respectively, with signs of congestive failure at the latter time point. Myosin isoform expression was examined in the subendocardial and subepicardial myocardium of the left ventricle and the free wall of the right ventricle by use of native gel electrophoresis. The percentage of the V3 isoform increased dramatically in both ventricles. In the subendocardial myocardium of the left ventricle, expression of the V3 isoform increased (p less than or equal to 0.05) relative to the subepicardial myocardium at 2, 4, and 8 weeks (17.1% vs. 10.2%, 29.4% vs. 18%, and 46.6% vs. 36.2%). In addition to regional differences within a given transmural segment, we also observed a high degree of heterogeneity in myosin isoform expression throughout a given layer (particularly the subendocardial myocardium) when small (less than or equal to 10-15 mg) adjacent samples were examined. This variability illustrated a potential danger in interpretation of gel results obtained from a single small tissue sample. Thus, cardiac hypertrophy produced by pressure overload in 25-day-old rats resulted in significantly increased V3 myosin in both the left and right ventricles. Furthermore, within the hypertrophied left ventricle, the subendocardial myocardium contained a significantly greater percentage of V3 myosin than the subepicardial myocardium.(ABSTRACT TRUNCATED AT 250 WORDS)
L B Bugaisky; P G Anderson; R S Hall; S P Bishop
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Circulation research     Volume:  66     ISSN:  0009-7330     ISO Abbreviation:  Circ. Res.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-05-09     Completed Date:  1990-05-09     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0047103     Medline TA:  Circ Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1127-32     Citation Subset:  IM    
Department of Pathology, University of Alabama, Birmingham 35294.
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MeSH Terms
Aorta, Thoracic
Cardiomegaly / enzymology*,  etiology
Electrophoresis / methods
Endocardium / enzymology*
Heart Failure / etiology
Myosins / metabolism*
Pericardium / enzymology*
Grant Support
Reg. No./Substance:

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