| Differences in magnetically induced motion of diamagnetic, paramagnetic, and superparamagnetic microparticles detected by cell tracking velocimetry. | |
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MedLine Citation:
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PMID: 19082082 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Magnetic separation in biomedical applications is based on differential magnetophoretic mobility (MM) of microparticulate matter in viscous media. Typically, the difference in MM is obtained by selectively labeling the target cells with superparamagnetic iron oxide nanoparticles (SPIONs). We have measured the MM of monodisperse, polystyrene microspheres (PSMs), with and without attached SPIONs as a model of cell motion induced by nanoparticle magnetization, using variable H field and cell tracking velocimetry (CTV). As a model of paramagnetic microparticle motion, the MM measurements were performed on the same PSMs in paramagnetic gadolinium solutions, and on spores of a prokaryotic organism, Bacillus globigii (shown to contain paramagnetic manganese). The CTV analysis was sensitive to the type of the microparticle magnetization, producing a value of MM independent of the applied H field for the paramagnetic species, and a decreasing MM value with an increasing field for superparamagnetic species, as predicted from theory. The SPION-labeled PSMs exhibited a saturation magnetization above H approximately = 64,000 A m(-1) (or 0.08 tesla). Based on those data, the average saturation magnetizations of the SPIONs was calculated and shown to vary between different commercial sources. The results demonstrate sensitivity of the CTV analysis to different magnetization mechanisms of the microparticles. |
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Authors:
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Xiaoxia Jin; Yang Zhao; Aaron Richardson; Lee Moore; P Stephen Williams; Maciej Zborowski; Jeffrey J Chalmers |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-09-09 |
Journal Detail:
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Title: The Analyst Volume: 133 ISSN: 1364-5528 ISO Abbreviation: Analyst Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-16 Completed Date: 2009-06-11 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0372652 Medline TA: Analyst Country: England |
Other Details:
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Languages: eng Pagination: 1767-75 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, OH 43210, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacillus / physiology* Magnetics* Manganese* Microspheres Movement Rheology / methods |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA062349-08/CA/NCI NIH HHS; R01 CA097391-01A1/CA/NCI NIH HHS; R01 CA97391A1/CA/NCI NIH HHS; R01CA62349/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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7439-96-5/Manganese |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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