| Differences in the forskolin activation of adenylate cyclases in wild-type and variant lymphoma cells. | |
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MedLine Citation:
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PMID: 6296654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability of the diterpene forskolin to stimulate cyclic AMP accumulation in intact cell and membrane preparations of wild-type S49 lymphoma cells (WT) and a number of variants has been confirmed. Additionally, a number of salient new findings have emerged: (a) A time delay in forskolin stimulation of cyclic AMP accumulation and adenylate cyclase (t 1/2 approximately equal to 1.5 min) occurred in all hormone-sensitive WT and variant cell and membrane preparations tested. (b) The time delay was missing in the adenylate cyclase-deficient variant (cyc-) of the S49 lymphoma cell, which also lacks functional adenylate cyclase-coupling proteins. (c) The simultaneous addition of epinephrine and forskolin to WT cells or to membrane preparations eliminated the time delay. (d) Forskolin stimulation of intact WT cells did not appear to desensitize adenylate cyclase. (e) The activation of WT adenylate cyclase by forskolin was biphasic with respect to concentration, with both high- and low-affinity components being apparent. In cyc-, only the low-affinity component was detected. |
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Authors:
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R B Clark; T J Goka; D A Green; R Barber; R W Butcher |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular pharmacology Volume: 22 ISSN: 0026-895X ISO Abbreviation: Mol. Pharmacol. Publication Date: 1982 Nov |
Date Detail:
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Created Date: 1983-03-24 Completed Date: 1983-03-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0035623 Medline TA: Mol Pharmacol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 609-13 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenylate Cyclase
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metabolism* Animals Cell Membrane / metabolism Cells, Cultured Cyclic AMP / metabolism Diterpenes / pharmacology* Enzyme Activation / drug effects Epinephrine / pharmacology Forskolin Lymphoma / metabolism* Neoplasms, Experimental / metabolism Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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AM 26943-03/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Diterpenes; 51-43-4/Epinephrine; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; EC 4.6.1.1/Adenylate Cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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