| Differences in follicular morphology, steroidogenesis and oocyte maturation in naturally cyclic and PMSG/hCG-treated prepubertal gilts. | |
| | |
MedLine Citation:
|
PMID: 2401990 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Ovaries were obtained from naturally cyclic pigs on Days 16-17, 18, 19, 20 and 21 of the oestrous cycle and on the basis of observed follicular characteristics were assigned as representative of the early (Group 1), mid- (Groups 2 and 3) or late (after LH; Group 4) follicular phase. Follicular development in cyclic gilts was compared with that in ovaries obtained from late prepubertal gilts 36 (Group 5) or 72 (Group 6) h after treatment with 750 i.u. PMSG alone, or with a combination of 500 i.u. hCG 72 h after PMSG and slaughter 30-40 h later (Group 7). After dissection of all follicles greater than 2 mm diameter, follicular diameter, follicular fluid volume, follicular fluid concentrations of progesterone, oestradiol and testosterone, and the stage of oocyte maturation were determined. Combined PMSG/hCG treatment of immature gilts resulted in a pattern of follicular development different from that in naturally cyclic gilts during the follicular phase. Overall exogenous gonadotrophin treatment also increased (P less than 0.001) the variability in follicular diameter and fluid volume. Comparisons between appropriate groups also established differences in the variability of both morphological (diameter and volume, Group 1 vs Group 5; P less than 0.05) and biochemical development (follicular fluid oestradiol, Group 3 vs Group 6 and Group 4 vs Group 7; both P less than 0.05). Such differences in both morphological and biochemical characteristics between cyclic and PMSG/hCG-treated gilts were particularly evident in the population of larger (greater than 6 mm) follicles. These results indicate that the pattern of follicular development in naturally cyclic and in PMSG/hCG-treated gilts is dissimilar and suggests that the ovaries of gonadotrophin-treated prepubertal gilts are functionally different from the ovaries of mature females. |
| | |
Authors:
|
T Wiesak; M G Hunter; G R Foxcroft |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of reproduction and fertility Volume: 89 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1990 Jul |
Date Detail:
|
Created Date: 1990-10-24 Completed Date: 1990-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 633-41 Citation Subset: IM |
Affiliation:
|
AFRC Research Group on Hormones and Farm Animal Reproduction, University of Nottingham, Faculty of Agricultural and Food Science, Sutton Bonington, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chorionic Gonadotropin / pharmacology* Estradiol / analysis Female Follicular Fluid / analysis Follicular Phase / physiology Gonadotropins, Equine / pharmacology* Ovarian Follicle / drug effects, physiology Ovary / physiology* Progesterone / analysis Sexual Maturation / physiology* Swine / physiology* Testosterone / analysis |
| Chemical | |
Reg. No./Substance:
|
0/Chorionic Gonadotropin; 0/Gonadotropins, Equine; 50-28-2/Estradiol; 57-83-0/Progesterone; 58-22-0/Testosterone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Immunosuppressive properties of follicular fluid from preovulatory horse follicles.
Next Document: Pitted pattern in the human epididymis.