Document Detail


Differences in expression and activity of beta,beta'-carotene-15,15'- oxygenase in liver and duodenum of cattle with yellow or white fat.
MedLine Citation:
PMID:  17615006     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pasture-fed cattle show yellow pigmentation of their fat due to beta-carotene stored in this tissue. beta,beta'-Carotene-15,15'-oxygenase (betaCO) is an enzyme expressed in different tissues, and it cleaves beta-carotene into retinal. We compared the expression and activity of betaCO in duodenum and liver of cattle with pigmented or non-pigmented fat. In the duodenum, in situ hybridizations showed expression of betaCO in epithelial cells and crypts of the mucosa that was similar in animals from pigmented and non-pigmented fat; liver showed diffuse signal at lobules, but pigmented animals showed higher signals near the portal space. Analyses by real-time reverse-transcription polymerase chain reaction also showed amplification of mRNA for betaCO in duodenum and liver, with no difference between pigmented or non-pigmented animals. Enzyme activity was similar in the duodenum, but pigmented animals had higher enzyme activity (p = 0.004) in liver. Cattle with pigmented fat had higher expression and activity of betaCO in liver, but its level was not high enough to prevent the storage of beta-carotene in adipose tissues.
Authors:
A Morales; A González; A Varela-Echavarría; A Shimada; O Mora
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of animal physiology and animal nutrition     Volume:  91     ISSN:  0931-2439     ISO Abbreviation:  J Anim Physiol Anim Nutr (Berl)     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-07-06     Completed Date:  2007-09-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101126979     Medline TA:  J Anim Physiol Anim Nutr (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  341-6     Citation Subset:  IM    
Affiliation:
Facultad de Estudios Superiores-Cuautitlán, Universidad Nacional Autónoma de México, Queretaro, Qro, Mexico.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / chemistry,  metabolism*
Animal Feed*
Animal Nutritional Physiological Phenomena
Animals
Cattle
Duodenum / enzymology*
Gene Expression Regulation, Enzymologic
In Situ Hybridization
Liver / enzymology*
Pigmentation
Poaceae
Reverse Transcriptase Polymerase Chain Reaction / veterinary
beta Carotene / administration & dosage,  metabolism
beta-Carotene 15,15'-Monooxygenase / genetics,  metabolism*
Chemical
Reg. No./Substance:
7235-40-7/beta Carotene; EC 1.14.99.36/beta-Carotene 15,15'-Monooxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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