| Differences in early and late responses between neurotrophin-stimulated trkA- and trkC-transfected SH-SY5Y neuroblastoma cells. | |
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MedLine Citation:
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PMID: 11205744 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Despite their sympathetic neuroblast origin, highly malignant neuroblastoma tumors and derived cell lines have no or low expression of the neurotrophin receptor genes, trkA and trkC. Expression of exogenous trkA in neuroblastoma cells restores their ability to differentiate in response to nerve growth factor (NGF). Here we show that stable expression of trkC in SH-SY5Y neuroblastoma cells resulted in morphological and biochemical differentiation upon treatment with neurotrophin-3 (NT-3). To some extent, trkA- and trkC-transfected SH-SY5Y (SH-SY5Y/trkA and SH-SY5Y/trkC) cells resembled one another in terms of early signaling events and neuronal marker gene expression, but important differences were observed. Although induced Erk 1/2 and Akt/PKB phosphorylation was stronger in NT-3-stimulated SH-Y5Y/trkC cells, activation of the immediate-early genes tested was more prominent in NGF-treated SH-SY5Y/ trkA cells. In particular, c-fos was not induced in the SH-SY5Y/trkC cells. There were also phenotypic differences. The concentrations of norepinephrine, the major sympathetic neurotransmitter, and growth cone-located synaptophysin, a neurosecretory granule protein, were increased in NGF-treated SH-SY5Y/trkA but not in NT-3-treated SH-SY5Y/trkC cells. Our data suggest that NT-3/p145trkC and NGF/p140trkA signaling differ in some aspects in neuroblasoma cells, and that this may explain the phenotypic differences seen in the long-term neurotrophin-treated cells. |
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Authors:
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A Edsjö; B Hallberg; S Fagerström; C Larsson; H Axelson; S Påhlman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research Volume: 12 ISSN: 1044-9523 ISO Abbreviation: Cell Growth Differ. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-02-02 Completed Date: 2001-03-29 Revised Date: 2012-06-22 |
Medline Journal Info:
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Nlm Unique ID: 9100024 Medline TA: Cell Growth Differ Country: United States |
Other Details:
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Languages: eng Pagination: 39-50 Citation Subset: IM |
Affiliation:
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Department of Laboratory Medicine, Lund University, University Hospital MAS, Malmö, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Northern Cell Differentiation Dose-Response Relationship, Drug Electrophoresis, Polyacrylamide Gel Fibroblast Growth Factors / metabolism Humans Immunoblotting Microscopy, Phase-Contrast Mitogen-Activated Protein Kinase 3 Mitogen-Activated Protein Kinases / metabolism Nerve Growth Factor / metabolism, pharmacology Nerve Growth Factors / metabolism* Neuroblastoma / metabolism* Neurons / metabolism Neurotrophin 3 / metabolism* Norepinephrine / biosynthesis Phenotype Phosphorylation Protein-Serine-Threonine Kinases* Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins c-akt Proto-Oncogene Proteins c-fos / biosynthesis RNA / metabolism Receptor, trkA / genetics*, metabolism Receptor, trkC / genetics*, metabolism Signal Transduction Synaptophysin / biosynthesis, metabolism Time Factors Transfection Tumor Cells, Cultured ras Proteins / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Nerve Growth Factors; 0/Neurotrophin 3; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-fos; 0/Synaptophysin; 51-41-2/Norepinephrine; 62031-54-3/Fibroblast Growth Factors; 63231-63-0/RNA; 9061-61-4/Nerve Growth Factor; EC 2.7.10.1/Receptor, trkA; EC 2.7.10.1/Receptor, trkC; EC 2.7.11.1/AKT1 protein, human; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 3.6.5.2/ras Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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