Document Detail


Differences in the composition of intestinal Bifidobacterium species and the development of allergic diseases in infants in rural Japan.
MedLine Citation:
PMID:  17430346     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Bifidobacterium is a dominant genus in the intestinal microbiota of infants and comprises many different species. A series of studies performed in northern Europe showed differences in the composition of Bifidobacterium species between allergic infants and healthy controls. Additional studies are needed to confirm this observation. OBJECTIVE: To investigate the composition of fecal Bifidobacterium species in allergic infants and healthy controls in Japan, using molecular methods. METHODS: Full-term born babies were followed up to 6 months of age at a local hospital in rural Japan. The presence or absence of allergy was determined based on allergic symptoms and skin prick tests. Fecal Bifidobacterium species in allergic infants (n=10), and healthy controls (n=16) were evaluated using nine Bifidobacterium species-specific or group-specific primers based on 16S rDNA sequences at 1, 3, and 6 months of age. RESULTS: The number of the infants in whom no Bifidobacterium species could be found was four (15.4%) at 1 month, two (7.7%) at 3 months, and one (3.3%) at 6 months of age, all of whom were healthy controls. At 1 month of age, allergic infants had a higher prevalence f the Bifidobacterium catenulatum group than healthy controls (60.0% vs. 6.3%, P<0.01). At 6 months of age, allergic infants had a higher prevalence of B. bifidum than healthy controls (70.0% vs. 12.5%, P<0.01). These differences were not related to feeding method. CONCLUSIONS: Our results in infants in rural Japan support the hypothesis that a compositional difference in intestinal Bifidobacterium species may be associated with the development of allergy in early infancy, although the responsible species might vary among countries or races.
Authors:
S Suzuki; N Shimojo; Y Tajiri; M Kumemura; Y Kohno
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology     Volume:  37     ISSN:  0954-7894     ISO Abbreviation:  Clin. Exp. Allergy     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-04-13     Completed Date:  2007-09-27     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8906443     Medline TA:  Clin Exp Allergy     Country:  England    
Other Details:
Languages:  eng     Pagination:  506-11     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Graduate School of Medicine Chiba University, Inohana, Chuo-ku, Chiba, Japan.
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MeSH Terms
Descriptor/Qualifier:
Bifidobacterium / classification*,  genetics,  isolation & purification
Feces / microbiology
Female
Follow-Up Studies
Humans
Hypersensitivity / immunology*
Infant
Infant Nutritional Physiological Phenomena
Intestines / microbiology*
Japan
Male
Polymerase Chain Reaction / methods
RNA, Bacterial / genetics
RNA, Ribosomal, 16S / genetics
Rural Health / statistics & numerical data
Skin Tests
Chemical
Reg. No./Substance:
0/RNA, Bacterial; 0/RNA, Ribosomal, 16S

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