Document Detail


Differences in claudin synthesis in primary cultures of acinar cells from rat salivary gland are correlated with the specific three-dimensional organization of the cells.
MedLine Citation:
PMID:  17347813     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Tight junctions are essential for the maintenance of epithelial cell polarity. We have previously established a system for the primary culture of salivary parotid acinar cells that retain their ability to generate new secretory granules and to secrete proteins in a signal-dependent manner. Because cell polarity and cell-cell adhesion are prerequisites for the formation of epithelial tissues, we have investigated the structure of the tight junctions in these cultures. We have found two types of cellular organization in the culture: monolayers and semi-spherical clusters. Electron microscopy has revealed tight junctions near the apical region of the lateral membranes between cells in the monolayers and cells at the surface of the clusters. The cells in the interior of the clusters also have tight junctions and are organized around a central lumen. These interior cells retain more secretory granules than the surface or monolayer cells, suggesting that they maintain their original character as acinar cells. The synthesis of claudin-4 increases during culture, although it is not detectable in the cells immediately after isolation from the glands. Immunofluorescence microscopy has shown that claudin-4 is synthesized in the monolayers and at the surface of the clusters, but not inside the clusters. Only claudin-3, which is present in the original acinar cells following isolation and in the intact gland, has been detected inside the clusters. These results suggest that differences in claudin expression are related to the three-dimensional structures of the cell cultures and reflect their ability to function as acinar cells.
Authors:
Bing Qi; Junko Fujita-Yoshigaki; Hiromi Michikawa; Keitaro Satoh; Osamu Katsumata; Hiroshi Sugiya
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-03-09
Journal Detail:
Title:  Cell and tissue research     Volume:  329     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-05-02     Completed Date:  2007-10-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  59-70     Citation Subset:  IM    
Affiliation:
Department of Physiology, Nihon University School of Dentistry at Matsudo, Sakaecho-nishi 2-870-1, Matsudo, Chiba, 271-8587, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Polarity / physiology*
Cells, Cultured
Epithelial Cells / metabolism*,  ultrastructure
Male
Membrane Proteins / biosynthesis*
Parotid Gland / metabolism*,  ultrastructure
Rats
Rats, Sprague-Dawley
Secretory Vesicles / metabolism*,  ultrastructure
Signal Transduction / physiology
Tight Junctions / metabolism*,  ultrastructure
Chemical
Reg. No./Substance:
0/Membrane Proteins; 0/claudin 3; 0/claudin 4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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