| Differences in the circular dichroism spectra of of eu- and heterochromatin fractions from rat liver. | |
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MedLine Citation:
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PMID: 450710 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Significant differences are observed between the CD spectra of eu- and heterochromatin prepared by fractionating mechanically sheared rat liver chromatin on linear sucrose density gradients. Heterochromatin has a broad positive CD at 270--280 nm, a cross-over point at 256 nmn, and a negative shoulder at 248 nm. Euchromatin displays a shift in the positive peak to 267 nm and the cross-over point to 254 nm, as well as an intensification of the 267 nm peak. The negative shoulder at 248 nm is absent in euchromatin. These spectral differences between eu- and heterochromatin are present in both low and high ionic strength media. Mechanical shearing does not induce CD alteration and anomalous light scattering is absent in our system. The greater RNA content of euchromatin compared to heterochromatin cannot account for the spectral differences. These results suggest that the DNA conformation of euchromatin is distinct from that of heterochromatin and they may provide clues to the reasons for the greater transcription of euchromatin. |
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Authors:
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G H Moyer; E Kay; G E Austin |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nucleic acids research Volume: 6 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1979 Apr |
Date Detail:
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Created Date: 1979-08-16 Completed Date: 1979-08-16 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1657-68 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Centrifugation, Density Gradient Chromatin / ultrastructure* Circular Dichroism DNA* / analysis Liver / ultrastructure* Nucleic Acid Conformation Nucleoproteins / analysis RNA / analysis Rats |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Nucleoproteins; 63231-63-0/RNA; 9007-49-2/DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Month: 4 Year: 1979 Volume: 6 Issue: 4 First Page: 1657 Last Page: 1668 ID: 327798 PubMed Id: 450710 |
| Differences in the circular dichroism spectra of of eu- and heterochromatin fractions from rat liver. | |
| G H Moyer | |
| E Kay | |
| G E Austin | |
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