Document Detail

Differences in centromere positioning of cycling and postmitotic human cell types.
MedLine Citation:
PMID:  15197559     Owner:  NLM     Status:  MEDLINE    
Centromere positioning in human cell nuclei was traced in non-cycling peripheral blood lymphocytes (G0) and in terminally differentiated monocytes, as well as in cycling phytohemagglutinin-stimulated lymphocytes, diploid lymphoblastoid cells, normal fibroblasts, and neuroblastoma SH-EP cells using immunostaining of kinetochores, confocal microscopy and three-dimensional image analysis. Cell cycle stages were identified for each individual cell by a combination of replication labeling with 5-bromo-2'-deoxyuridine and immunostaining of pKi67. We demonstrate that the behavior of centromeres is similar in all cell types studied: a large fraction of centromeres are in the nuclear interior during early G1; in late G1 and early S phase, centromeres shift to the nuclear periphery and fuse in clusters. Peripheral location and clustering of centromeres are most pronounced in non-cycling cells (G0) and terminally differentiated monocytes. In late S and G2, centromeres partially decluster and migrate towards the nuclear interior. In the rather flat nuclei of adherently growing fibroblasts and neuroblastoma cells, kinetochores showed asymmetrical distributions with preferential kinetochore location close either to the bottom side of the nucleus (adjacent to the growth surface) or to the nuclear upper side. This asymmetrical distribution of centromeres is considered to be a consequence of chromosome arrangement in anaphase rosettes.
Irina Solovei; Lothar Schermelleh; Klaus Düring; Andrea Engelhardt; Stefan Stein; Christoph Cremer; Thomas Cremer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-06-09
Journal Detail:
Title:  Chromosoma     Volume:  112     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  2004 Jun 
Date Detail:
Created Date:  2004-06-18     Completed Date:  2005-01-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  410-23     Citation Subset:  IM    
Department of Biology II, Humangenetik, Ludwig Maximillians University (LMU), Richard Wagner Str. 10, 80333, Munich, Germany.
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MeSH Terms
Bromodeoxyuridine / diagnostic use
Cell Cycle / genetics*
Cell Differentiation
Cell Nucleus / genetics*
Centromere / genetics*
DNA Replication
Fibroblasts / cytology
Lymphocytes / cytology*
Mitosis / genetics*
Monocytes / cytology
Neuroblastoma / pathology
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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