Document Detail


Differences in capillary electrophoresis profiles of urinary and recombinant erythropoietin.
MedLine Citation:
PMID:  12601737     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Different profiles were obtained by capillary zone electrophoresis (CZE) of human erythropoietin (EPO) of recombinant and urinary origin. To unambiguously detect doping by EPO, direct methods able to determine the presence of the drug itself in a physiological fluid are required. Since the host cell line used for EPO production influences its glycosylation, the carbohydrate distribution of natural human EPO may be different from that of recombinant EPO. The different content in sialic acid groups between recombinant and endogenous EPO provide a basis for their distinction by CZE.
Authors:
Mercedes De Frutos; Alejandro Cifuentes; Jose C Diez-Masa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Electrophoresis     Volume:  24     ISSN:  0173-0835     ISO Abbreviation:  Electrophoresis     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-25     Completed Date:  2003-11-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8204476     Medline TA:  Electrophoresis     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  678-80     Citation Subset:  IM    
Affiliation:
Institute of Organic Chemistry, CSIC, Madrid, Spain. mfrutos@iqog.csic.es
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MeSH Terms
Descriptor/Qualifier:
Electrophoresis, Capillary / methods*
Erythropoietin / urine*
Erythropoietin, Recombinant / urine*
Humans
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Erythropoietin, Recombinant; 11096-26-7/Erythropoietin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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