| Differences in amplification efficiency of standard curves in quantitative real-time PCR assays and consequences for gene quantification in environmental samples. | |
| | |
MedLine Citation:
|
PMID: 20621132 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
High and comparable efficiency values are the key for reliable quantification of target genes from environmental samples using real-time PCR. Therefore it was the aim of this study to investigate if PCR amplification efficiencies of plasmid DNA used for the calculation of standard curves (i) remain constant along a logarithmic scale of dilutions and (ii) if these values are comparable to those of DNA extracted from environmental samples. It could be shown that comparable efficiency values within the standards cannot be achieved using log scale serial dilutions and a comparison of gene copy numbers from DNA extracted from environmental samples and standard DNA extracted from plasmids is only possible in a very small interval. |
| | |
Authors:
|
Stefanie Töwe; Kristina Kleineidam; Michael Schloter |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-15 |
Journal Detail:
|
Title: Journal of microbiological methods Volume: 82 ISSN: 1872-8359 ISO Abbreviation: J. Microbiol. Methods Publication Date: 2010 Sep |
Date Detail:
|
Created Date: 2010-08-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8306883 Medline TA: J Microbiol Methods Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 338-41 Citation Subset: IM |
Copyright Information:
|
(c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
|
Technische Universität München, Chair of Soil Ecology, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany. stefanie.toewe@helmholtz-muenchen.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: UPLC/MS based method for quantitative determination of fatty acid composition in gram-negative and g...
Next Document: Rapid sporulation of Bacillus anthracis in a high iron, glucose-free medium.