Document Detail

Differences between karyotypically normal and abnormal human embryonic stem cells.
MedLine Citation:
PMID:  20546238     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: To compare different biological characteristics of human embryonic stem cells (HESCs) between those with normal and those with abnormal karyotype. MATERIALS AND METHODS: Culture-adapted HESCs (chHES-3) with abnormal karyotype were compared with karyotypically normal cells, with regard to pluripotency and differentiation capacity, ultrastructure, growth characteristics, gene expression profiles and signalling pathways. RESULTS: We found a new abnormal karyotype of HESCs. We observed that chHES-3 cells with normal and abnormal karyotypes shared similarities in expression markers of pluripotency; however, karyotypically abnormal chHES-3 cells had a tendency for differentiation towards ectoderm lineages and were easily maintained in suboptimal culturing conditions. Abnormal chHES-3 cells displayed relatively mature cell organelles compared to normal cells, and karyotypically abnormal chHES-3 cells had increased survival and population growth. Genes related to cell proliferation and apoptosis were up-regulated, but genes associated with genetic instability (p53, Rb, BRCA1) were down-regulated in the karyotypically abnormal cells. CONCLUSION: Karyotypically abnormal chHES-3 cells had a more developed capacity for proliferation, resistance to apoptosis and less genetic stability compared to normal chHES-3 cells and may be an excellent model for studying and characterizing initial stages that determine transition of embryonic stem cells into cancer stem cells.
S Yang; G Lin; Y-Q Tan; L-Y Deng; D Yuan; G-X Lu
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell proliferation     Volume:  43     ISSN:  1365-2184     ISO Abbreviation:  Cell Prolif.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-15     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  195-206     Citation Subset:  IM    
Institute of Reproductive and Stem Cell Engineering, Central South University, National Engineering Research Center of Human Stem Cells, Changsha, P. R. China.
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MeSH Terms
Cell Differentiation / genetics*
Cell Line
Cell Lineage / genetics*
Cell Proliferation
Cell Survival / genetics
Cell Transformation, Neoplastic / genetics
Cells, Cultured
Chromosomal Instability / genetics
Chromosome Aberrations*
Ectoderm / metabolism,  ultrastructure
Embryonic Stem Cells / metabolism*,  ultrastructure*
Gene Expression Regulation / genetics
Mutation / genetics*
Organelles / metabolism,  ultrastructure
Tumor Suppressor Proteins / genetics
Reg. No./Substance:
0/Tumor Suppressor Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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