Document Detail


Differences between karyotypically normal and abnormal human embryonic stem cells.
MedLine Citation:
PMID:  20546238     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: To compare different biological characteristics of human embryonic stem cells (HESCs) between those with normal and those with abnormal karyotype. MATERIALS AND METHODS: Culture-adapted HESCs (chHES-3) with abnormal karyotype were compared with karyotypically normal cells, with regard to pluripotency and differentiation capacity, ultrastructure, growth characteristics, gene expression profiles and signalling pathways. RESULTS: We found a new abnormal karyotype of HESCs. We observed that chHES-3 cells with normal and abnormal karyotypes shared similarities in expression markers of pluripotency; however, karyotypically abnormal chHES-3 cells had a tendency for differentiation towards ectoderm lineages and were easily maintained in suboptimal culturing conditions. Abnormal chHES-3 cells displayed relatively mature cell organelles compared to normal cells, and karyotypically abnormal chHES-3 cells had increased survival and population growth. Genes related to cell proliferation and apoptosis were up-regulated, but genes associated with genetic instability (p53, Rb, BRCA1) were down-regulated in the karyotypically abnormal cells. CONCLUSION: Karyotypically abnormal chHES-3 cells had a more developed capacity for proliferation, resistance to apoptosis and less genetic stability compared to normal chHES-3 cells and may be an excellent model for studying and characterizing initial stages that determine transition of embryonic stem cells into cancer stem cells.
Authors:
S Yang; G Lin; Y-Q Tan; L-Y Deng; D Yuan; G-X Lu
Related Documents :
8293548 - Genotoxicity of tamoxifen, tamoxifen epoxide and toremifene in human lymphoblastoid cel...
2556928 - Origin and relationship between different cell types in malignant fibrous histiocytoma.
6260908 - The morphologic response of cell lines derived from human gliomas to dibutyryl adenosin...
1204748 - Establishment of normal diploid and malignant heteroploid cell lines from non-treated a...
24668208 - The impact of vitrification on immature oocyte cell cycle and cytoskeletal integrity in...
20956378 - The cell biology of touch.
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell proliferation     Volume:  43     ISSN:  1365-2184     ISO Abbreviation:  Cell Prolif.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-15     Completed Date:  2010-06-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  195-206     Citation Subset:  IM    
Affiliation:
Institute of Reproductive and Stem Cell Engineering, Central South University, National Engineering Research Center of Human Stem Cells, Changsha, P. R. China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / genetics*
Cell Line
Cell Lineage / genetics*
Cell Proliferation
Cell Survival / genetics
Cell Transformation, Neoplastic / genetics
Cells, Cultured
Chromosomal Instability / genetics
Chromosome Aberrations*
Ectoderm / metabolism,  ultrastructure
Embryonic Stem Cells / metabolism*,  ultrastructure*
Gene Expression Regulation / genetics
Humans
Mice
Mutation / genetics*
Organelles / metabolism,  ultrastructure
Tumor Suppressor Proteins / genetics
Chemical
Reg. No./Substance:
0/Tumor Suppressor Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Switching from a preserved to a preservative-free prostaglandin preparation in topical glaucoma medi...
Next Document:  Induction of bone formation by transforming growth factor-beta in the non-human primate Papio ursinu...