Document Detail


Differences between the O-acetylated sialic acids of the epithelial mucins of human colonic tumors and normal controls: a correlative chemical and histochemical study.
MedLine Citation:
PMID:  7354217     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chemical analyses, together with histochemical assessments, were carried out on specimens of adenocarcinoma of the colon and histologically normal colonic epithelium (from resection margins from cases of carcinoma of the colon). In the epithelial glycoproteins of the normal tissue, both chemical and histochemical investigations indicated that the great majority of the sialic acids contained a side-chain O-acyl substituent located at position C8, whereas the side-chain substitution of the sialic acids of tumor glycoproteins was markedly reduced. Chemical analysis of the normal glycoproteins indicated that the great majority of the sialic acids were resistant to digestion with Vibrio cholerae neuraminidase, presumably due to an ester substituent at C4. The sialic acids of the tumor glycoproteins were significantly different from normal, in that they were less resistant to digestion with neuraminidase (p greater than 0.01), and therefore had a lower percentage of substitution at C4 (p greater than 0.01).
Authors:
P E Reid; C F Culling; W L Dunn; C W Ramey; A B Magil; M G Clay
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  28     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1980 Mar 
Date Detail:
Created Date:  1980-04-23     Completed Date:  1980-04-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  217-22     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / analysis*
Chemical Phenomena
Chemistry
Colon / analysis*
Colonic Neoplasms / analysis*
Epithelium / analysis
Glycoproteins / analysis
Histocytochemistry
Humans
Mucins / analysis*
Neoplasm Proteins / analysis
Neuraminidase / pharmacology
Sialic Acids / analysis*
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Mucins; 0/Neoplasm Proteins; 0/Sialic Acids; EC 3.2.1.18/Neuraminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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