Document Detail


Difference in uptake of junctional epithelium using microperoxidase and horseradish peroxidase as tracers in healthy rat gingiva
MedLine Citation:
PMID:  2519254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A microperoxidase (MP, molecular weight 1,900; molecular diameter 20 A) or a horseradish peroxidase (HRP, molecular weight 40,000; molecular diameter 40 A) was intravenously injected into healthy rat junctional epithelium (JE) to investigate the endocytosis of foreign substances. By light microscopy, intravenous HRP was taken up throughout the JE, and the uptake was marked at the coronal portion of the JE. On the other hand, MP was taken up by only the coronal portion of the JE. At the electron microscopic level, the tracers were taken up by endocytotic organellae (phagosomes and micropinocytotic vesicles) of junctional epithelial cells (JE cells). HRP-positive endocytotic organellae in the JE cells were more numerous than MP-positive organellae. Thus, JE, especially its coronal portion, exhibited strong endocytotic activity for HRP compared with that for MP. These findings suggest that the JE has selective endocytotic ability for foreign substances, and plays an important role in protecting periodontal tissue.
Authors:
N Ayasaka; H Nakamura; T Tanaka
Publication Detail:
Type:  English Abstract; Journal Article    
Journal Detail:
Title:  Shika Kiso Igakkai zasshi = Japanese journal of oral biology     Volume:  31     ISSN:  0385-0137     ISO Abbreviation:  Shika Kiso Igakkai Zasshi     Publication Date:  1989 Feb 
Date Detail:
Created Date:  1991-11-22     Completed Date:  1991-11-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7506047     Medline TA:  Shika Kiso Igakkai Zasshi     Country:  JAPAN    
Other Details:
Languages:  jpn     Pagination:  61-71     Citation Subset:  D    
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MeSH Terms
Descriptor/Qualifier:
Animals
Endocytosis*
Epithelial Attachment / enzymology,  metabolism*
Gingiva / metabolism*
Horseradish Peroxidase / metabolism
Peroxidases / metabolism
Rats
Chemical
Reg. No./Substance:
EC 1.11.1.-/Horseradish Peroxidase; EC 1.11.1.-/Peroxidases; EC 1.11.1.-/microperoxidase

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