Document Detail


Difference in sensitivity of inner cell mass and trophectoderm to X-irradiation in mouse blastocysts.
MedLine Citation:
PMID:  9587968     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pregnant B6C3F1 mice were exposed to a single whole body X-irradiation on day 4 (73-74 hr postconception) of gestation. In experiment 1, they were sacrificed at 2, 4, 6, or 9 hr after a dose of 2 Gy, and their embryos were removed and examined with light and electron microscopy. In experiment 2, dose-response effects of irradiation on the embryos were examined 4 hr after doses of 0-4 Gy. In experiment 3, DNA fragmentation (a marker of apoptosis) was observed by 3'-OH nick-end labeling technique. In inner cell mass (ICM) and trophectoderm (TE) of blastocysts exposed to 2 Gy, cells with cytoplasmic degeneration, or dead cells phagocytosed by their neighboring cells, were found. Although morphological features of these dying cells did not reveal typical characteristics of apoptosis such as nuclear condensation and membrane blebbing, DNA fragmentation was detected by nick-end labeling technique. The degenerated cytoplasm consisted of aggregating ribosomes. Degenerated cells began to increase from 2 hr after irradiation and reached maximal at 4 hr in both ICM and TE. The incidences of degenerated cells in ICM were higher than those in TE at any time point. These findings provide evidence that cell death observed in blastocysts after X-irradiation is apoptotic and sensitivity of the two groups of cells (ICM and TE) to X-rays is different.
Authors:
T Kumazawa; M Inouye; I Hayasaka; H Yamamura; Y Murata
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Teratology     Volume:  57     ISSN:  0040-3709     ISO Abbreviation:  Teratology     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-07-14     Completed Date:  1998-07-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0153257     Medline TA:  Teratology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  146-51     Citation Subset:  IM    
Affiliation:
Department of Teratology and Genetics, Research Institute of Environmental Medicine, Nagoya University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis / radiation effects
Blastocyst / cytology*,  radiation effects*
DNA Damage / radiation effects*
Ectoderm / radiation effects
Female
Mice
Pregnancy

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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