| Difference in phototoxicity of cyclodextrin complexed fullerene [(gamma-CyD)2/C60] and its aggregated derivatives toward human lens epithelial cells. | |
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MedLine Citation:
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PMID: 19281132 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The water-soluble fullerene derivative gamma-cyclodextrin bicapped C(60) [(gamma-CyD)(2)/C(60), CDF0] has several clinical applications, including use as a drug carrier to bypass the blood ocular barriers or a photosensitizer to treat tumors in photodynamic therapy. We have assessed the potential ocular toxicity of (gamma-CyD)(2)/C(60) and its aggregated derivatives induced by UVA and visible light in vitro in human lens epithelial cells (HLE B-3). Cell viability using the MTS assay demonstrated that 2 microM (gamma-CyD)(2)/C(60) was highly phototoxic to HLE B-3 cells with UVA irradiation, while no effect was observed in the presence of visible light or when maintained in the dark. In contrast, the aggregated derivative (CDF150) showed neither cytotoxicity nor any phototoxic effect even at 30 microM with either UVA or visible light irradiation. In lens cells treated with (gamma-CyD)(2)/C(60), phototoxicity was manifested as apoptosis. Singlet oxygen production measurement using the EPR/TEMP trapping technique determined that (gamma-CyD)(2)/C(60) (CDF0) efficiently produced singlet oxygen. The rate of singlet oxygen production decreased with increased aggregation, with no production by the fully aggregated sample formed after 150 min of heating (CDF150). UVA irradiation of HLE B-3 in the presence of (gamma-CyD)(2)/C(60) resulted in a significant rise in intracellular protein-derived peroxides. The singlet oxygen quenchers sodium azide and histidine each significantly protected lens cells against (gamma-CyD)(2)/C(60) photodamage, but lutein and Trolox (vitamin E) did not. Clearly, singlet oxygen is an important intermediate in the phototoxicity of monomeric (gamma-CyD)(2)/fullerene. Our results also demonstrate that UVA-blocking sunglasses can limit the ocular phototoxicity of this nanomaterial, while nontoxic endogenous antioxidants like lutein or Trolox cannot provide adequate protection. |
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Authors:
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Baozhong Zhao; Yu-Ying He; Colin F Chignell; Jun-Jie Yin; Usha Andley; Joan E Roberts |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural |
Journal Detail:
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Title: Chemical research in toxicology Volume: 22 ISSN: 1520-5010 ISO Abbreviation: Chem. Res. Toxicol. Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-04-20 Completed Date: 2009-07-07 Revised Date: 2012-03-08 |
Medline Journal Info:
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Nlm Unique ID: 8807448 Medline TA: Chem Res Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: 660-7 Citation Subset: IM |
Affiliation:
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Laboratory of Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Electron Spin Resonance Spectroscopy Epithelial Cells / drug effects* Fullerenes / pharmacology, toxicity* Humans Lens, Crystalline / cytology, drug effects* Peroxidases / metabolism Photosensitizing Agents / pharmacology, toxicity* Singlet Oxygen / metabolism Temperature Ultraviolet Rays gamma-Cyclodextrins / pharmacology, toxicity* |
| Grant Support | |
ID/Acronym/Agency:
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Z99 ES999999/ES/NIEHS NIH HHS; ZIA ES102225-03/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/(gamma-CyD)2 C60; 0/Fullerenes; 0/Photosensitizing Agents; 0/gamma-Cyclodextrins; 17465-86-0/gamma-cyclodextrin; 17778-80-2/Singlet Oxygen; EC 1.11.1.-/Peroxidases |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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