Document Detail

Difference in the localization of tetrodotoxin between the female and male pufferfish Takifugu niphobles, during spawning.
MedLine Citation:
PMID:  22842064     Owner:  NLM     Status:  Publisher    
In order to understand the sexual differences in TTX-usage in the pufferfish, Takifugu niphobles, localization of TTX and toxin amount in tissues of mature male and female specimens were investigated by immunohistochemical methods using anti-TTX antibody and LC/MS analysis. Subsequently, differences in the immunohistochemical signals were compared with the amount of TTX. The paraffin-embedded sections of the skin, muscle, liver, gonad and intestinal tract were subjected to anti-TTX monoclonal antibody based on the fluorescent immunohistochemical techniques. Immuno-positive reaction was observed in the skin and liver in males, and the skin and ovary in females. In the skin, TTX was localized at the epidermis, the basal cell layer, the mucous cells and the sacciform cells, and with intense immunoreaction at the flat epithelial cell layer and the sacciform cells. The signal from the liver cells was stronger in males than in females. The intensity of the signal from the tissues correlated with the toxin amounts therein. These results suggest that tissue distributions of TTX and toxin amount in the pufferfish were sex-dependent.
Shiro Itoi; Saori Yoshikawa; Ryohei Tatsuno; Miwa Suzuki; Kiyoshi Asahina; Shota Yamamoto; Shihori Takanashi; Tomohiro Takatani; Osamu Arakawa; Yoshitaka Sakakura; Haruo Sugita
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-27
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  -     ISSN:  1879-3150     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-31     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier Ltd.
Department of Marine Science and Resources, Nihon University, Fujisawa, Kanagawa 252-0880, Japan.
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