Document Detail

Dietary supplementation with cholesterol and docosahexaenoic acid increases the activity of the arginine-nitric oxide pathway in tissues of young pigs.
MedLine Citation:
PMID:  18555806     Owner:  NLM     Status:  MEDLINE    
Nitric oxide (NO), synthesized from l-arginine by tetrahydrobiopterin (BH4)-dependent NO synthase (NOS), is critical for neurological and muscular development and function. This study was designed to test the hypothesis that cholesterol and docosahexaenoic acid (DHA) may modulate the arginine-NO pathway in tissues of the young pig. Sixteen newborn pigs were nursed by sows for 24h and then assigned to one of four treatment groups, representing supplementation with 0.0%, 0.2% cholesterol, 0.2% DHA, or cholesterol plus DHA to the basal milk-formula. All piglets were euthanized at 49 days of age. Brain, liver and gastrocnemius muscle were analyzed for BH4, NADPH and arginine, GTP cyclohydrolase-I (GTP-CH) and NOS activities, and NOS protein isoforms. Hepatic NOS activity was below the detection limit in all pigs. DHA supplementation (P<0.01) increased GTP-CH activities, as well as BH4 and NADPH concentrations in brain, liver, and muscle by 24-46%, while enhancing (P<0.05) NOS activities by 45-48% in brain and muscle. Dietary cholesterol supplementation increased (P<0.05) NOS and GTP-CH activities by 17-26% in brain but had no effect in liver or muscle. The enhanced NOS activity in the brain or muscle of cholesterol- or DHA-supplemented piglets was attributable to the combined effects of increased eNOS and nNOS activation (changes in phosphorylation levels) and total iNOS protein. Additionally, DHA and cholesterol enhanced (P<0.05) arginine concentrations in brain (35-42%), but not in liver or muscle. These tissue-specific effects of cholesterol and DHA on NO synthesis may play an important role in postnatal growth and development.
Peng Li; Sung Woo Kim; Xilong Li; Sujay Datta; Wilson G Pond; Guoyao Wu
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Publication Detail:
Type:  Journal Article     Date:  2008-05-27
Journal Detail:
Title:  Nitric oxide : biology and chemistry / official journal of the Nitric Oxide Society     Volume:  19     ISSN:  1089-8611     ISO Abbreviation:  Nitric Oxide     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-17     Completed Date:  2008-12-22     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  9709307     Medline TA:  Nitric Oxide     Country:  United States    
Other Details:
Languages:  eng     Pagination:  259-65     Citation Subset:  IM    
Department of Animal Science and Faculty of Nutrition, Texas A&M University, College Station, TX 77843-2471, USA.
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MeSH Terms
Analysis of Variance
Animals, Newborn
Arginine / metabolism*
Biopterin / analogs & derivatives,  metabolism
Blotting, Western
Brain / drug effects,  metabolism
Cholesterol, Dietary / administration & dosage*
Chromatography, High Pressure Liquid
Citrulline / metabolism
Dietary Supplements*
Docosahexaenoic Acids / administration & dosage*
GTP Cyclohydrolase / metabolism
Liver / drug effects,  metabolism
Muscle, Skeletal / drug effects,  metabolism
NADP / metabolism
Nitric Oxide / metabolism*
Nitric Oxide Synthase / metabolism
Grant Support
R21 HD049449-01A2/HD/NICHD NIH HHS
Reg. No./Substance:
0/Cholesterol, Dietary; 10102-43-9/Nitric Oxide; 17528-72-2/5,6,7,8-tetrahydrobiopterin; 22150-76-1/Biopterin; 25167-62-8/Docosahexaenoic Acids; 372-75-8/Citrulline; 53-59-8/NADP; 74-79-3/Arginine; EC Oxide Synthase; EC Cyclohydrolase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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