| Dietary soy protein isolate attenuates metabolic syndrome in rats via effects on PPAR, LXR, and SREBP signaling. | |
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MedLine Citation:
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PMID: 19515742 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine the effects of feeding soy or isoflavones on lipid homeostasis in early development, weanling rats were fed AIN-93G diets made with casein, soy protein isolate (SPI+), isoflavone-reduced SPI+ (SPI-), or casein supplemented with genistein or daidzein for 14 d. PPARalpha-regulated genes and proteins involved in fatty acid degradation were upregulated by SPI+ (P < 0.05) accompanied by increased promoter binding and expression of PPARalpha mRNA (P < 0.05). Feeding SPI- or pure isoflavones did not alter PPARalpha-regulated pathways. SPI+ feeding had similar effects on PPARgamma signaling. SPI+, SPI-, and casein plus isoflavones all increased liver X-receptor (LXR)alpha-regulated genes and enzymes involved in cholesterol homeostasis. Feeding SPI+ increased promoter binding of LXRalpha, expression of the transcription factor mRNA, and protein (P < 0.05). In a second experiment, male Sprague-Dawley rats were fed casein diets from postnatal d (PND) 24 to PND64 or were fed high-fat Western diets containing 5 g x kg(-1) cholesterol made with either casein or SPI+. Insulin resistance, steatosis, and hypercholesterolemia in the Western diet-fed rats were partially prevented by SPI+ (P < 0.05). Nuclear sterol receptor element binding protein (SREBP)-1c protein and mRNA and protein expression of enzymes involved in fatty acid synthesis were increased by feeding Western diets containing casein but not SPI+ (P < 0.05). These data suggest that activation of PPAR and LXR signaling and inhibition of SREBP-1c signaling may contribute to insulin sensitization and improved lipid homeostasis in SPI+-fed rats after consumption of diets high in fat and cholesterol. |
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Authors:
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Martin J Ronis; Ying Chen; Jamie Badeaux; Thomas M Badger |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-10 |
Journal Detail:
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Title: The Journal of nutrition Volume: 139 ISSN: 1541-6100 ISO Abbreviation: J. Nutr. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-07-21 Completed Date: 2009-08-13 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 1431-8 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Toxicology, University of Arkansas for Medical Sciences, Little Rock, AR 72202, USA. ronismartinj@uams.edu <ronismartinj@uams.edu> |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticholesteremic Agents / pharmacology, therapeutic use Caseins / administration & dosage, pharmacology Cholesterol / metabolism DNA-Binding Proteins / genetics, metabolism* Dietary Fats Fatty Liver / prevention & control Female Gene Expression / drug effects Genistein / pharmacology, therapeutic use Hypercholesterolemia / prevention & control Insulin Resistance Isoflavones / pharmacology, therapeutic use* Lipid Metabolism / drug effects* Male Metabolic Syndrome X / drug therapy, genetics, metabolism* Orphan Nuclear Receptors PPAR alpha / genetics, metabolism* PPAR gamma / metabolism Promoter Regions, Genetic / drug effects RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Receptors, Cytoplasmic and Nuclear / genetics, metabolism* Signal Transduction / drug effects Soybean Proteins / pharmacology, therapeutic use* Sterol Regulatory Element Binding Protein 1 / metabolism* Weight Gain / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Anticholesteremic Agents; 0/Caseins; 0/DNA-Binding Proteins; 0/Dietary Fats; 0/Isoflavones; 0/Orphan Nuclear Receptors; 0/PPAR alpha; 0/PPAR gamma; 0/RNA, Messenger; 0/Receptors, Cytoplasmic and Nuclear; 0/Soybean Proteins; 0/Sterol Regulatory Element Binding Protein 1; 0/liver X receptor; 446-72-0/Genistein; 486-66-8/daidzein; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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