| Dietary soy may not confound acute experimental stroke infarct volume outcomes in ovariectomized female rats. | |
| | |
MedLine Citation:
|
PMID: 20147341 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Oestrogen administration can alter experimental stroke outcomes. Soy as a source of phytoestrogens may therefore modulate responses in 'oestrogen-sensitive' stroke models, thus potentially confounding results. We evaluated the effects of dietary soy on acute infarct volumes in a pilot study using a rat focal stroke model. We hypothesized that ovariectomized (OVX) rats fed a soy-rich diet would have smaller acute infarct volumes than rats fed a soy-free diet. OVX rats were randomly assigned to a soy-free (n = 6) or a soy-rich (n = 6) diet for four weeks and weighed weekly. Following the dietary trial, rats underwent 2 h of middle cerebral artery occlusion (MCAO). Mean arterial blood pressure, rectal and temporalis muscle temperatures, arterial blood gases and blood glucose were recorded peri-ischaemia. Rats were euthanized 22 h following 2 h of MCAO. Brains were stained with 2,3,5-triphenyl tetrazolium chloride for acute infarct volume analysis. Uterine weight and histology were also evaluated as additional internal oestrogen-sensitive controls. Rats on the soy-free diet had greater gains in body weight (259 +/- 6% baseline body weight) than rats on the soy-rich diet (238 +/- 4% baseline body weight). No differences were seen in uterine weight and histology, peri-ischaemic physiological parameters and infarct volumes between the treatment groups. The results of this pilot study suggest that the dietary soy level tested may not alter acute infarct volumes in ischaemic female rat brain. More studies addressing the potential confounding effects of dietary soy in 'oestrogen-sensitive' stroke models are needed if investigators are to make informed choices regarding diets used in experimental stroke research. |
| | |
Authors:
|
Kamm D Prongay; Anne D Lewis; Patricia D Hurn; Stephanie J Murphy |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-10 |
Journal Detail:
|
Title: Laboratory animals Volume: 44 ISSN: 1758-1117 ISO Abbreviation: Lab. Anim. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-07-01 Completed Date: 2010-09-28 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 0112725 Medline TA: Lab Anim Country: England |
Other Details:
|
Languages: eng Pagination: 238-46 Citation Subset: IM |
Affiliation:
|
Department of Comparative Medicine, Oregon Health and Science University, Portland, OR 97239, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animal Feed Animals Blood Gas Analysis Blood Glucose / analysis Blood Pressure Body Temperature Body Weight / drug effects Brain / drug effects*, pathology Confounding Factors (Epidemiology) Disease Models, Animal Female Infarction, Middle Cerebral Artery / complications, drug therapy*, pathology Muscle, Skeletal / drug effects, physiopathology Organ Size / drug effects Ovariectomy Rats Rats, Wistar Soybeans / adverse effects* Stroke / complications, drug therapy*, pathology Uterus / drug effects, pathology |
| Grant Support | |
ID/Acronym/Agency:
|
P01 NS049210-029002/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Blood Glucose |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effects of intra-cage aspen tube on cardiac morphology and gene expression.
Next Document: Coinheritance of severe von Willebrand disease with Glanzmann thrombasthenia.