| Dietary selenium (Se) and copper (Cu) interact to affect homocysteine metabolism in rats. | |
| | |
MedLine Citation:
|
PMID: 19104759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Previously, we reported that both Se deficiency and Cu deficiency decreased plasma homocysteine (pHcys) and increased plasma glutathione (pGSH) in rats. We also showed that the catalytic subunit of glutamate-cysteine ligase (Gclc), which catalyzes the rate-limiting step in glutathione biosynthesis, was upregulated in Se and Cu deficiencies. We suggested that in both deficiencies, Hcys was being shunted through the trans-sulfuration pathway as a result of this up-regulation. Because both Se and Cu deficiencies have similar effects, we hypothesized that a combined deficiency would exacerbate the decrease in pHcys and the increase in pGSH by further up-regulating Gclc. In a 2 x 2 experiment, male weanling Sprague-Dawley rats (n = 8-20/group) were fed an amino-acid-based diet containing either 0 or 0.2 microg Se (as selenite)/g and <1 or 6 microg Cu (as Cu carbonate)/g for 5 weeks. Our findings show that a combined deficiency of both Se and Cu results in lower pHcys and significantly elevated pGSH. However, the up-regulation of liver Gclc alone cannot explain why rats fed with the doubly deficient diet have the lowest pHcys and the highest pGSH. |
| | |
Authors:
|
Eric O Uthus; Sharon Ross |
Related Documents
:
|
3243699 - The effect of a low copper diet on blood cholesterol and enzymic antioxidant defense me... 2087449 - Broiler chicks fed low-calcium diets. 2. increased sensitivity to copper toxicity. 7769369 - Hemostatic mechanisms in marginally copper-deficient rats. 8002459 - Stimulation of growth by intravenous injection of copper in weanling pigs. 22282049 - Long-term beneficial effect of a 16-week very low calorie diet on pericardial fat in ob... 7899209 - Effect of high copper dosing on hemicellulose digestibility in cecectomized cockerels. 7109209 - Dietary risk factors of stroke and hypertension in japan -- part 1: methodological asse... 12560759 - Dietary fat attenuates the benefits of an elemental diet in active crohn's disease: a r... 19620679 - Efficacy of solis, novasilplus, and mtb-100 to reduce aflatoxin m1 levels in milk of ea... |
Publication Detail:
|
Type: Journal Article Date: 2008-12-23 |
Journal Detail:
|
Title: Biological trace element research Volume: 129 ISSN: 1559-0720 ISO Abbreviation: Biol Trace Elem Res Publication Date: 2009 |
Date Detail:
|
Created Date: 2009-05-22 Completed Date: 2009-07-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7911509 Medline TA: Biol Trace Elem Res Country: United States |
Other Details:
|
Languages: eng Pagination: 213-20 Citation Subset: IM |
Affiliation:
|
Grand Forks Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Grand Forks, ND 58202-9034, USA. eric.uthus@ars.usda.gov |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animal Feed* Animals Copper / administration & dosage*, deficiency, pharmacology* Drug Interactions Glutathione / blood Liver / chemistry, drug effects, metabolism Male Rats Rats, Sprague-Dawley S-Adenosylhomocysteine / chemistry, metabolism* S-Adenosylmethionine / chemistry, metabolism Selenium / administration & dosage*, deficiency, pharmacology* |
| Chemical | |
Reg. No./Substance:
|
29908-03-0/S-Adenosylmethionine; 70-18-8/Glutathione; 7440-50-8/Copper; 7782-49-2/Selenium; 979-92-0/S-Adenosylhomocysteine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Determination of copper and iron in biological samples of viral hepatitis (A-E) female patients.
Next Document: Expression and Purification of Human Keratinocyte Growth Factor 2 by Fusion with SUMO.