| Dietary proteins extend the survival of salmonella dublin in a gastric Acid environment. | |
| | |
MedLine Citation:
|
PMID: 22289597 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
The pH of the human stomach is dynamic and changes over time, depending on the composition of the food ingested and a number of host-related factors such as age. To evaluate the number of bacteria surviving the gastric acid barrier, we have developed a simple gastric acid model, in which we mimicked the dynamic pH changes in the human stomach. In the present study, model gastric fluid was set up to imitate pH dynamics in the stomachs of young and elderly people after ingestion of a standard meal. To model a serious foodborne pathogen, we followed the survival of Salmonella enterica serotype Dublin, and found that the addition of proteins such as pepsin, ovalbumin, and blended turkey meat to the simple gastric acid model significantly delayed pathogen inactivation compared with the control, for which no proteins were added. In contrast, no delay in inactivation was observed in the presence of bovine serum albumin, indicating that protection could be protein specific. The simple gastric acid model was validated against a more laborious and complex fermenter model, and similar survival of Salmonella Dublin was observed in both models. Our gastric acid model allowed us to evaluate the influence of food components on survival of pathogens under gastric conditions, and the model could contribute to a broader understanding of the impact of specific food components on the inactivation of pathogens during gastric passage. |
| | |
Authors:
|
Tina Birk; Kim Kristensen; Anne Harboe; Tina Beck Hansen; Hanne Ingmer; Rob De Jonge; Katsuhisa Takumi; Søren Aabo |
Related Documents
:
|
832337 - Effects of alpha-tocopherol analogs on lysosome membranes and fatty acid monolayers. 3878307 - The inactivation of plasma alpha 1-proteinase inhibitor by nitrous acid. 17068257 - Alpha-hydroxy and alpha-amino acids under possible hadean, volcanic origin-of-life cond... 7280887 - The effect of seromucoid on coagulation. 16045947 - Composition of fatty acids triacylglycerols and unsaponifiable matter in calophyllum ca... 18383017 - Use of quasi-isoelectric buffers as anolyte and catholyte to improve capillary isoelect... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of food protection Volume: 75 ISSN: 1944-9097 ISO Abbreviation: J. Food Prot. Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-01-31 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
|
Languages: eng Pagination: 353-8 Citation Subset: IM |
Affiliation:
|
National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, 2860 Søborg, Denmark. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Rapid detection of salmonella in pet food: design and evaluation of integrated methods based on real...
Next Document: Investigating hydrophobicity and the effect of exopolysaccharide on aggregation properties of dairy ...