| Dietary protein hydrolysates and free amino acids affect the spatial expression of peptide transporter PepT1 in the digestive tract of Atlantic cod (Gadus morhua). | |
| | |
MedLine Citation:
|
PMID: 20144729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The effects of dietary inclusions of size-fractionated peptides and free amino acids (FAAs) on Peptide Transporter 1 (PepT1) mRNA levels were assessed along the length of the intestine of juvenile Atlantic cod (Gadus morhua). Five groups of fish (10-15g) were fed for 46days on diets containing approximately 42% protein, provided either as fish meal (FM, control diet) or as a combination of FM with whole fish hydrolysate (FH), retenate after ultrafiltration of FH (UFR), nanofiltered retenate of FH (NFR), or a mix of FAAs, at a 30% level of FM substitution. PepT1 mRNA expression was assessed in pyloric caeca (S1) and the remainder of the intestine divided into four equally long segments (S2-S5). PepT1 transcripts were found in all segments, indicating that the whole intestine is involved in peptide absorption. Differences in the regional expression profile of PepT1 were found. Under control diet (FM diet) conditions, fish exhibited a reduced expression in S5 compared to S2. In fish fed FAA and UFR diets, PepT1 mRNA levels were higher in S2 and S3 compared to other regions. These data suggest that PepT1 may be variably recruited along the whole intestine, including the most distal part, in response to changes in the luminal protein source content. This adaptive response might be functional to keep a maximal efficiency of protein absorption at the intestinal level. |
| | |
Authors:
|
Snorre Bakke; Ann-Elise Olderbakk Jordal; Pedro G?mez-Requeni; Tiziano Verri; Katerina Kousoulaki; Anders Aksnes; Ivar R?nnestad |
Related Documents
:
|
17030189 - Cd36 is important for chylomicron formation and secretion and may mediate cholesterol u... 15539269 - Effects of dietary polyamines and clofibrate on metabolism of polyamines in the rat. 996439 - Jejunitis with malaborption. a quantitative study of the gastric and intestinal handlin... 6669929 - Whole-gut transit time and its relationship to absorption of macronutrients during diar... 6972889 - Regional blood flow and the localisation of lymphoblasts in the small intestine of the ... 15590889 - Involvement of mrp2 in hepatic and intestinal disposition of dinitrophenyl-s-glutathion... 12656649 - Lovastatin extended release: a review of its use in the management of hypercholesterola... 15066369 - Walking, lipids, and lipoproteins: a meta-analysis of randomized controlled trials. 1322879 - Effects of protein and energy supplementation of wheat straw-based diets on site of nut... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-06 |
Journal Detail:
|
Title: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology Volume: 156 ISSN: 1879-1107 ISO Abbreviation: Comp. Biochem. Physiol. B, Biochem. Mol. Biol. Publication Date: 2010 May |
Date Detail:
|
Created Date: 2010-03-22 Completed Date: 2010-06-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9516061 Medline TA: Comp Biochem Physiol B Biochem Mol Biol Country: England |
Other Details:
|
Languages: eng Pagination: 48-55 Citation Subset: IM |
Copyright Information:
|
2010 Elsevier Inc. All rights reserved. |
Affiliation:
|
Department of Biology, University of Bergen, Norway. snorre@mfaa.no |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
metabolism* Animals Gadus morhua / genetics, metabolism* Gastrointestinal Tract / metabolism* Gene Expression Regulation Oligopeptides / metabolism Protein Hydrolysates / metabolism* RNA, Messenger / metabolism Symporters / genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Oligopeptides; 0/PepT1 protein; 0/Protein Hydrolysates; 0/RNA, Messenger; 0/Symporters |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum.
Next Document: Metabolic changes in a pyruvate kinase gene deletion mutant of Corynebacterium glutamicum ATCC 13032...