| Dietary protein differentially regulates the kinetic behaviour of serine dehydratase and tyrosine aminotransferase of liver and white muscle of rainbow trout (Oncorhynchus mykiss). | |
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MedLine Citation:
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PMID: 20601064 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have determined the kinetic behaviour of serine dehydratase (SerDH) and tyrosine aminotransferase (TyrAT) in the liver and white muscle of juvenile rainbow trout (Oncorhynchus mykiss) fed on a low-protein/high-fat (LP/HF) and control diets. The relationship between the kinetic parameters and tissue-DNA concentration has also been determined. SerDH and TyrAT showed hyperbolic kinetics in all cases. The partial replacement of protein with fat significantly increased hepatic SerDH-specific activity, maximum velocity (V(max)) and Michaelis constant (K(m)) while no significant changes were detected in the values of these parameters in white muscle. Nevertheless, hepatic and white-muscle TyrAT specific activity and V(max) decreased in trout fed on LP/HF diet with respect to control. The K(m) of hepatic TyrAT was also lower in trout fed on LP/HF diet than in control. The TyrAT kinetic parameters expressed per cell unit also changed in the same sense as previously described. These results show that, in trout, SerDH and TyrAT are two enzymes regulated by the partial replacement of dietary protein by fat. |
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Authors:
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Juan Peragón; Manuel De La Higuera; José A Lupiáñez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-23 |
Journal Detail:
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Title: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology Volume: 157 ISSN: 1879-1107 ISO Abbreviation: Comp. Biochem. Physiol. B, Biochem. Mol. Biol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-02 Completed Date: 2010-09-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9516061 Medline TA: Comp Biochem Physiol B Biochem Mol Biol Country: England |
Other Details:
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Languages: eng Pagination: 213-9 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Biochemistry and Molecular Biology Section, Department of Experimental Biology, University of Jaén, Jaén, Spain. jperagon@ujaen.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Dietary Proteins / administration & dosage* Kinetics L-Serine Dehydratase / metabolism* Liver / enzymology Muscles / enzymology Oncorhynchus mykiss / growth & development, metabolism* Tyrosine Transaminase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Dietary Proteins; EC 2.6.1.5/Tyrosine Transaminase; EC 4.3.1.17/L-Serine Dehydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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