Document Detail

Dietary polyunsaturated fatty acids modulate in vivo, antigen-driven CD4+ T-cell proliferation in mice.
MedLine Citation:
PMID:  15284386     Owner:  NLM     Status:  MEDLINE    
Our objective was to determine whether dietary lipids affect in vivo, antigen-driven, proliferation of naïve CD4(+) T lymphocytes. To accomplish this, we adoptively transferred lymphocytes from T-cell receptor (TCR) transgenic DO11.10 (i.e., donor) mice into syngeneic, nontransgenic BALB/c (i.e., recipient) mice. Before adoptive transfer, recipient mice were fed for 4 wk AIN93G-type diets that differed only in fat source: lard, low in PUFA, fish oil, rich in (n-3) PUFA, and soybean oil, rich in (n-6) PUFA. One week after transfer, recipient mice were immunized with antigen (i.e., ovalbumin), and expansion of CD4(+) T(DO11.10) cells in the spleen and draining lymph nodes (LN) was measured by flow cytometry. Five days postimmunization (p.i.), at the peak of expansion, CD4(+) T(DO11.10) cells in the draining LN and spleen were 5- to 10-fold higher than in unimmunized mice, then quickly declined during the contraction phase (i.e., 7 and 10 d p.i.). Recipients fed the (n-6) PUFA rich diet had approximately 25% greater in vivo expansion of CD4(+) T(DO11.10) cells than lard- and fish oil-fed recipient mice at 5 d p.i. (P < 0.05). However, at 7 and 10 d p.i., CD4(+) T(DO11.10) cells in the draining lymph nodes did not differ between groups, nor in the spleen at 5, 7, and 10 d p.i. In summary, we are the first to demonstrate that dietary PUFAs affect antigen-driven expansion of naïve CD4(+) T cells in vivo. Surprisingly, (n-3) PUFA consumption did not reduce CD4(+) T-cell expansion.
Michele J Anderson; Kevin L Fritsche
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of nutrition     Volume:  134     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-07-30     Completed Date:  2004-08-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1978-83     Citation Subset:  IM    
Department of Animal Sciences, University of Missouri, Columbia, MO 65211, USA.
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MeSH Terms
Animals, Genetically Modified
Antigens, CD4 / blood,  drug effects*
Dietary Fats / administration & dosage,  pharmacology*
Fatty Acids, Unsaturated / administration & dosage,  pharmacology*
Mice, Inbred BALB C
Receptors, Antigen, T-Cell / drug effects*
Spleen / drug effects,  immunology
Reg. No./Substance:
0/Antigens, CD4; 0/Dietary Fats; 0/Fatty Acids, Unsaturated; 0/Receptors, Antigen, T-Cell

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